A newly developed means of HPLC-fluorescence analysis for predicting the skin sensitization potential of multi-constituent substances using ADRA.

Authors:
Masaharu Fujita
Masaharu Fujita
Fujifilm Corporation
Bangkok | Thailand
Yusuke Yamamoto
Yusuke Yamamoto
Shizuoka Cancer Center
Japan
Toshihiko Kasahara
Toshihiko Kasahara
University of Shizuoka
Japan

Toxicol In Vitro 2019 Apr 17;59:161-178. Epub 2019 Apr 17.

Safety Evaluation Centre, Ecology & Quality Management Division, CSR Division, FUJIFILM Corporation, 210 Nakanuma, Minamiashigara-shi, Kanagawa, Japan.

The Amino acid Derivative Reactivity Assay (ADRA) is an in chemico alternative to animal testing for skin sensitization potential, in which measurements of multi-constituent solutions were sometimes affected by co-elution with nucleophilic reagents. So, we established a means of using fluorescence detection and verified the utility of a newly developed ADRA-fluorescence detection (ADRA-FL) test method. We tested three types of plant extracts-aloe, green tea, and licorice-and although unable to quantify nucleophilic reagents using ultraviolet detection due to co-elution of multiple components, the use of fluorescence detection enabled us to detect nucleophilic reagents selectively and predict each of the extract solutions to be sensitizers. Given that plant extracts contain immunosuppressants, there is no reason to expect that positive results in ADRA-FL testing will always be concordant with in vivo results. But given its ability to predict the sensitization potential of cosmetics and other widely used multi-constituent substances that had previously been difficult to test, the newly developed ADRA-FL is expected to contribute to future assessments of sensitization risks.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tiv.2019.04.014DOI Listing
April 2019
1 Read

Publication Analysis

Top Keywords

nucleophilic reagents
12
sensitization potential
12
newly developed
12
multi-constituent substances
8
skin sensitization
8
fluorescence detection
8
detection adra-fl
4
developed adra-fluorescence
4
adra-fluorescence detection
4
adra-fl test
4
solutions sensitizers
4
test method
4
sensitizers plant
4
extracts immunosuppressants
4
plant extracts
4
utility newly
4
detection
4
established fluorescence
4
positive adra-fl
4
reagents established
4

Similar Publications