PM2.5 induces EMT and promotes CSC properties by activating Notch pathway in vivo and vitro.

Authors:
Yunxia Wang
Yunxia Wang
School of Public Health
New Haven | United States
Tianfang Hou
Tianfang Hou
Peking University First Hospital
Jiping Liao
Jiping Liao
Peking University First Hospital
China
Cheng Zhang
Cheng Zhang
Anhui Medical University
China
Chao Sun
Chao Sun
Institute of Medicinal Plant Development
China
Guangfa Wang
Guangfa Wang
Peking University First Hospital
China

Ecotoxicol Environ Saf 2019 Aug 16;178:159-167. Epub 2019 Apr 16.

Department of Respiratory and Critical Care Medicine, Peking University First Hospital, Beijing, China. Electronic address:

Fine particulate matter (PM2.5) has been closely linked to increased morbidity and mortality of lung cancer worldwide. However, the role of PM2.5 in the etiology of lung cancer and the mechanism involved in PM2.5 induced lung cancer are largely unknown. In this study, we performed chronic exposure animal model to investigate the carcinogenetic mechanisms of PM2.5 by targeting the induction of epithelial-mesenchymal transition (EMT) and cancer stem cells (CSC) properties through Notch1 signal pathway. The antagonism of Notch1 signal pathway was carried out in vitro cell lines of A549 and BEAS-2B to block EMT and CSC. We found that chronic PM2.5 exposure mice lung tissue pathology showed atypical hyperplasia of bronchiolar epithelium. Then, we discovered that chronic PM2.5 exposure induced notable EMT event and obvious CSC properties indicating the developing process of cell malignant behaviors. EMT characterized with decreased protein expression of E-cadherin and increased protein expression of Vimentin. CSC properties induced by chronic PM2.5 exposure characterized with increased cell-surface markers (ABCG2 and ALDH1A1) and self-renewal genes (SOX2 and OCT4). Furthermore, PM2.5 exposure activate Notch signal pathway by increasing expression of Notch1 and Hes1. At last, we blocked Notch signal pathway by inhibitor RO4929097 in vitro to explore the underlying mechanism mediating PM2.5 induced EMT and CSC. We found that blocking Notch1 could prevent PM2.5 induced malignant behaviors including EMT and CSC in A549 and BEAS-2B. These data revealed that the induction of EMT and CSC properties were involved in the lung cancer risk of PM2.5 in vivo, and blocking-up Notch1 may negatively regulate EMT and CSC to suppress the invasion and migration in vitro, thereby putatively serving as a novel therapeutic target for PM2.5 induced lung cancer.

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Source
https://linkinghub.elsevier.com/retrieve/pii/S01476513193035
Publisher Site
http://dx.doi.org/10.1016/j.ecoenv.2019.03.086DOI Listing
August 2019
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