Ecotoxicol Environ Saf 2019 Aug 16;178:130-136. Epub 2019 Apr 16.
Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan Province, 650500, China. Electronic address:
A simple, effective and convenient method for determination of phenolic compounds by acid-base induced deep eutectic solvents (DESs) microextraction was developed. The binary and ternary DESs were prepared by a range of fatty acids (C-C), which can act as hydrogen bond donors and hydrogen bond acceptors simultaneously. The gas-assisted mixing customization provides excellent mixing performance and concentration efficiency through the bubble adsorption mechanism for the handling of large-volume aqueous sample. In extraction process, NH·HO can act as the emulsifier agent and reacted with DESs to form salts with a cloudy solution, which can obviously improve the extraction efficiency. HCl can act as the phase separation agent, and there is no need to centrifuge, which increases the efficiency of analysis procedure. The factors affected on extraction efficiency were carefully optimized. At optimum conditions and molar ratio of C:C:C (3:2:1), the limit of detections (LODs), the preconcentration factor, the repeatability (RSDs%) were in the range of 0.22-0.53 μg L, 235-244, and 2.6-6.7%, respectively. Finally, the proposed method was applied to analyze four phenolic compounds in real water samples and the recoveries were between 87.4% and 106.6%.