Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: Characterization of four fatty acyl elongases and a novel desaturase capacity.

Authors:
Zhaoshou Ran
Zhaoshou Ran
Key Laboratory of Applied Marine Biotechnology
Jilin Xu
Jilin Xu
Ningbo University
China
Kai Liao
Kai Liao
Colorado State University
United States
Oscar Monroig
Oscar Monroig
Institute of Aquaculture
United Kingdom
Juan Carlos Navarro
Juan Carlos Navarro
Hospital General Universitario de Elche
Spain
Angela Oboh
Angela Oboh
Institute of Aquaculture
United Kingdom
Min Jin
Min Jin
State Key Laboratory Breeding Base of Marine Genetic Resource
China

Biochim Biophys Acta Mol Cell Biol Lipids 2019 Apr 16;1864(8):1083-1090. Epub 2019 Apr 16.

Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education of China, Ningbo, Zhejiang 315211, China. Electronic address:

As an unusual economically important aquaculture species, Sinonovacula constricta possesses high levels of long-chain polyunsaturated fatty acids (LC-PUFA). Previously, our group identified fatty acyl desaturases (Fad) with Δ5 and Δ6 activities in S. constricta, which was the first report of Δ6 Fad in a marine mollusc. Here, we further successfully characterize elongases of very long-chain fatty acids (Elovl) in this important bivalve species, including one Elovl2/5, two Elovl4 isoforms (a and b) and a novel Elovl (c) with Elovl4 activity. In addition, we also determined the desaturation activity of S. constricta Δ6 Fad toward 24:5n-3 to give 24:6n-3, a key intermediate in docosahexaenoic acid (DHA) biosynthesis. Therefore, S. constricta is the first marine mollusc reported to possess all Fad and Elovl activities required for LC-PUFA biosynthesis via the 'Sprecher pathway'. This finding greatly increases our understanding of LC-PUFA biosynthesis in marine molluscs. Phylogenetic analysis by interrogating six marine molluscan genomes, and previously functionally characterized Elovl and Fad from marine molluscs, suggested that DHA biosynthetic ability was limited to a few species, due to the general lack of Δ4 or Δ6 Fad in most molluscs.

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Source
https://linkinghub.elsevier.com/retrieve/pii/S13881981193005
Publisher Site
http://dx.doi.org/10.1016/j.bbalip.2019.04.004DOI Listing
April 2019
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