Long-term administration of Aspalathus linearis infusion affects spatial memory of adult Sprague-Dawley male rats as well as increases their striatal dopamine content.

Authors:
Justyna Pyrzanowska
Justyna Pyrzanowska
Medical University of Warsaw
Izabela Fecka
Izabela Fecka
Wrocław Medical University
Poland
Dagmara Mirowska-Guzel
Dagmara Mirowska-Guzel
Institute of Psychiatry and Neurology
Poland
Ilona Joniec-Maciejak
Ilona Joniec-Maciejak
Medical University of Warsaw
Poland
Kamilla Blecharz-Klin
Kamilla Blecharz-Klin
Medical University of Warsaw
Poland
Agnieszka Piechal
Agnieszka Piechal
Department of Experimental and Clinical Pharmacology
Poland

J Ethnopharmacol 2019 Jun 16;238:111881. Epub 2019 Apr 16.

Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Centre for Preclinical Research and Technology CePT, Banacha 1b, 02-097, Warsaw, Poland. Electronic address:

Ethnopharmacological Relevance: Everyday use of the herbal tea rooibos, produced from Aspalathus linearis (Brum.f) Dahlg. (Fabaceae) is customary in South Africa, a continuation of its historical use by indigenous people. Although evidence of its traditional indications is anecdotal, rooibos tea is regarded as a general health tea.

Aims Of The Study: Available contemporary research indicates to broad cell protective activity of rooibos focusing on its antioxidative, anti-inflammatory, anti-hyperglycaemic and antithrombotic features affecting metabolic syndrome, cardiovascular risk and neuroprotection. Nevertheless little is known about its impact on brain functions. The present experiment aimed to evaluate the possible behavioural and neurochemical effects of long-term oral administration of "fermented" rooibos herbal tea (FRHT) infusions to adult male Sprague-Dawley rats.

Materials And Methods: Infusions, prepared using 1, 2 and 4 g of "fermented" (oxidised) A. linearis leaves for 100 ml of hot water, were characterised in terms of flavonoid content by ultra-high and high performance liquid chromatography (UHPLC-qTOF-MS, HPLC-DAD) and administered to rats as sole drinking fluid for 12 weeks. Spatial memory behaviour was assessed in a modified version of the Morris water maze. Dopamine, noradrenaline, serotonin and their metabolite levels (DOPAC, 3-MT, HVA, MHPG, 5-HIAA) were quantified in prefrontal cortex, hippocampus and striatum by HPLC-ECD. Body weight and blood glucose level were additionally estimated.

Results: All FRHT-treated rats showed improvement of long-term spatial memory defined as increased number of crossings over the previous platform position in SE quadrant of the water maze. It was not accompanied by excessive motor activity. Striatal dopamine and its metabolite 3-MT (3-methoxytyramine) levels were increased in treated rats. There were no differences in body weight gain between control and treated animals but blood glucose level was significantly lower in the latter ones.

Conclusion: The improvement of long-term memory in FRHT-treated rats and stimulating impact of FRHT on their dopaminergic striatal transmission support the wellness enhancing effect of rooibos tea, contributing to a better understanding of the neurological background of traditional habitual consumption of this herbal tea.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jep.2019.111881DOI Listing
June 2019
1 Read

Publication Analysis

Top Keywords

spatial memory
12
herbal tea
12
striatal dopamine
8
improvement long-term
8
frht-treated rats
8
glucose level
8
water maze
8
rooibos tea
8
body weight
8
blood glucose
8
aspalathus linearis
8
tea
5
rooibos
5
rats
5
liquid chromatography
4
fluid weeks
4
weeks spatial
4
memory behaviour
4
assessed modified
4
behaviour assessed
4

Altmetric Statistics

Similar Publications