Bioactive sphingolipid profile in a xenograft mouse model of head and neck squamous cell carcinoma.

Authors:
Aiping Bai
Aiping Bai
Medical University of South Carolina
United States
Xiang Liu
Xiang Liu
Endoscopy Center
Tulsa | United States
Jacek Bielawski
Jacek Bielawski
Medical University of South Carolina
Charleston | United States
Yusuf A Hannun
Yusuf A Hannun
Stony Brook University
Stony Brook | United States

PLoS One 2019 19;14(4):e0215770. Epub 2019 Apr 19.

Departments of Medicine and Biochemistry & the Stony Brook Cancer Center at Stony Brook University, Stony Brook, New York, United States of America.

The purpose of this study was to determine the profile of bioactive sphingolipids in xenograft mouse tissues of head and neck squamous cell carcinoma. We utilized UHPLC-MS/MS to determine the profile of full set of ceramides, sphingosine, and sphingosine 1-phosphate in this xenograft mouse model. The tissues isolated and investigated were from brain, lung, heart, liver, spleen, kidney, bladder, tumors and blood. With the exception of equal volume of blood plasma (100ul), all tissues were studied with the same amount of protein (800ug). Results demonstrated that brain contained the highest level of ceramide and kidney had the highest level of sphingosine, whereas sphingosine 1-phosphate and dihydrosphingosine 1-phosphate were heavily presented in the blood. Brain also comprised the highest level of phospholipids. As for the species, several ceramides, usually present in very low amounts in cultured tumor cells, showed relatively high levels in certain tissues. This study highlights levels of bioactive sphingolipids profiles in xenograft mouse model of head and neck squamous cell carcinoma, and provides resources to investigate potential therapeutic targets and biomarkers that target bioactive sphingolipids metabolism pathways.

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Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215770PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6474618PMC
April 2019
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