Survival of Mycobacterium bovis BCG oral vaccine during transit through a dynamic in vitro model simulating the upper gastrointestinal tract of badgers.

Authors:
Gareth A Williams
Gareth A Williams
University of London
United Kingdom
Marjorie E Koenen
Marjorie E Koenen
Institute for Animal Science and Health
Netherlands
Robert Havenaar
Robert Havenaar
TNO
Netherlands
Paul Wheeler
Paul Wheeler
Veterinary Laboratories Agency (Weybridge)
Sonya Gowtage
Sonya Gowtage
Department of Bacteriology
Madison | United States
Sandrine Lesellier
Sandrine Lesellier
University College Dublin
Ireland
Mark A Chambers
Mark A Chambers
Veterinary Laboratories Agency Weybridge

PLoS One 2019 19;14(4):e0214859. Epub 2019 Apr 19.

Department of Bacteriology, Animal and Plant Health Agency, New Haw, Addlestone, United Kingdom.

In developing an oral bait BCG vaccine against tuberculosis in badgers we wanted to understand the conditions of the gastrointestinal tract and their impact on vaccine viability. Conditions mimicking stomach and small-intestine caused substantial reduction in BCG viability. We performed in vivo experiments using a telemetric pH monitoring system and used the data to parameterise a dynamic in vitro system (TIM-1) of the stomach and small intestine. Some BCG died in the stomach compartment and through the duodenum and jejunum compartments. BCG survival in the stomach was greatest when bait was absent but by the time BCG reached the jejunum, BCG viability was not significantly affected by the presence of bait. Our data suggest that from a starting quantity of 2.85 ± 0.45 x 108 colony-forming units of BCG around 2 log10 may be killed before delivery to the intestinal lymphoid tissue. There are economic arguments for reducing the dose of BCG to vaccinate badgers orally. Our findings imply this could be achieved if we can protect BCG from the harsh environment of the stomach and duodenum. TIM-1 is a valuable, non-animal model with which to evaluate and optimise formulations to maximise BCG survival in the gastrointestinal tract.

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214859PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6474584PMC
April 2019
1 Read

Publication Analysis

Top Keywords

gastrointestinal tract
12
bcg
11
bcg viability
8
dynamic vitro
8
bcg survival
8
stomach
5
lymphoid tissue
4
parameterise dynamic
4
data parameterise
4
vitro system
4
tim-1 stomach
4
small intestine
4
intestine bcg
4
delivery intestinal
4
stomach small
4
tissue economic
4
intestinal lymphoid
4
system tim-1
4
monitoring system
4
bcg vaccinate
4

References

(Supplied by CrossRef)
The Strategy for achieving Officially Bovine Tuberculosis Free status for England
Bacillus Calmette-Guerin vaccination reduces the severity and progression of tuberculosis in badgers
M Chambers et al.
Proceedings of the Royal Society B-Biological Sciences 2011
The Effect of Oral Vaccination with Mycobacterium bovis BCG on the Development of Tuberculosis in Captive European Badgers (Meles meles)
MA Chambers et al.
Frontiers in Cellular and Infection Microbiology 2017

Similar Publications