ESRRG promoter hypermethylation as a diagnostic and prognostic biomarker in laryngeal squamous cell carcinoma.

Authors:
Zhisen Shen
Zhisen Shen
Lihuili Hospital of Ningbo University
China
Yan Hu
Yan Hu
Chongqing University
China
Chongchang Zhou
Chongchang Zhou
The Medical School of Ningbo University
Jie Yuan
Jie Yuan
Sun Yat-sen University
China
Jie Xu
Jie Xu
College of Food Science and Engineering
Columbus | United States
Wenjuan Hao
Wenjuan Hao
Xuzhou Normal University
China
Hongxia Deng
Hongxia Deng
Ningbo University School of Medicine
China
Dong Ye
Dong Ye
Jinan University
China

J Clin Lab Anal 2019 Apr 19:e22899. Epub 2019 Apr 19.

Department of Otorhinolaryngology-Head and Neck Surgery, Lihuili Hospital of Ningbo University, Ningbo, China.

Background: Estrogen-related receptor gamma (ESRRG) has been identified as a tumor suppressor gene in several cancers. We aimed to evaluate ESRRG promoter methylation in laryngeal squamous cell carcinoma (LSCC) and its relative clinical value in LSCC.

Methods: Bisulfite pyrosequencing assays were performed on 91 pairs of tumor and paracancer tissues from LSCC patients in China. The diagnostic value and overall survival (OS) were analyzed descriptively by receiver operating characteristic (ROC) curves and the Kaplan-Meier methods, respectively.

Results: The ESRRG promoter was more frequently hypermethylated in tumor tissues than in adjacent tissues (P < 0.01). ESRRG promoter methylation was significantly increased in advanced T stage tumors (P < 0.01) and advanced clinical stage patients (P < 0.01). Moreover, the area under the ROC curve (AUC) value (0.81) indicated high discrimination accuracy. Furthermore, ESRRG hypermethylation was associated with poor OS, as confirmed by Kaplan-Meier survival curves (P < 0.01).

Conclusion: Our study indicated that ESRRG promoter hypermethylation contributed to LSCC-related risks, primarily tumor progression and survival prognosis, in patients. ESRRG promoter methylation could, therefore, be a diagnostic and prognostic biomarker in LSCC.

Download full-text PDF

Source
http://dx.doi.org/10.1002/jcla.22899DOI Listing
April 2019

Publication Analysis

Top Keywords

esrrg promoter
12
laryngeal squamous
8
squamous cell
8
cell carcinoma
8
roc curves
4
clinical lsccmethods
4
esrrg
4
bisulfite pyrosequencing
4
lsccmethods bisulfite
4
curves kaplan-meier
4
lscc relative
4
evaluate esrrg
4
methods respectivelyresults
4
cancers aimed
4
promoter methylation
4
methylation laryngeal
4
pyrosequencing assays
4
kaplan-meier methods
4
carcinoma lscc
4
relative clinical
4

References

(Supplied by CrossRef)
Cancer statistics, 2018
Siegel RL et al.
CA Cancer J Clin 2018
Global DNA methylation status in laryngeal cancer
Stembalska A et al.
Head Neck 2014
Cancer statistics, 2017
Siegel RL et al.
CA Cancer J Clin 2017

Similar Publications