Intelligent TAT-coupled anti-HER2 immunoliposomes knock downed MDR1 to produce chemosensitize phenotype of multidrug resistant carcinoma.

Authors:
Neda Gholamian Dehkordi
Neda Gholamian Dehkordi
University of Isfahan
Fatemeh Elahian
Fatemeh Elahian
School of Pharmacy
Chapel Hill | United States
Pegah Khosravian
Pegah Khosravian
Department of Pharmaceutics
Seyed Abbas Mirzaei
Seyed Abbas Mirzaei
School of Pharmacy

J Cell Physiol 2019 Apr 18. Epub 2019 Apr 18.

Department of Medical Biotechnology, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Gene therapy using biocompatible cationic liposomes is amongst promising approaches that decreases death from cancers. Here an invasive multidrug resistant cell model has been developed by lentiviral transfection. In parallel phospholipids have been covalently conjugated to TAT, MMP2, and Herceptin. The functional lipids have been mixed to generate intelligent liposome harboring small interfering RNA (siRNA) with high efficiency. The final liposomal complex was uniformly monodisperse and particle dimension and zeta-potential were respectively around 200 nm and -42.21 mV. Minimal cytotoxic effects have been reported for nanocarriers due to good biocompatibility of the selected phospholipids. Flourescence-activated cell sorter (FACS) analyses have been represented that surface trastuzumab and TAT specifically promote cellular uptake of liposomes in the malignant tumor cells. Assessment of MDR1 transcript and protein expression has been exhibited maximum significant downregulation around of 128-fold and 50-fold, respectively after 48 hr of liposome exposure. As it has been concluded, targeted liposomes may become a potential tool in gene delivery for improving chemotherapeutic efficiency in cancer treatment.

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.28683
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http://dx.doi.org/10.1002/jcp.28683DOI Listing
April 2019
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Journal of Liposome Research 2015

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