Antimicrobial Peptide JH-3 Effectively Kills Salmonella enterica Serovar Typhimurium Strain CVCC541 and Reduces Its Pathogenicity in Mice.

Authors:
Lei Wang
Lei Wang
West China Hospital
Nanchong Shi | China
Xueqin Zhao
Xueqin Zhao
College of Life Sciences
Pueblo | United States
Chunling Zhu
Chunling Zhu
State Key Laboratory of Genetic Resources and Evolution
Wanhai Qin
Wanhai Qin
Jilin University
China
Yanzhao Xu
Yanzhao Xu
Key Discipline Open Laboratory of Animal Viral Disease Control and Residual Analysis of Henan Province
Bolin Hang
Bolin Hang
Henan Institute of Science and Technology
China
Yawei Sun
Yawei Sun
Huazhong Agricultural University
China

Probiotics Antimicrob Proteins 2019 Apr 18. Epub 2019 Apr 18.

Postdoctoral Research Base, Henan Institute of Science and Technology, Xinxiang, People's Republic of China.

Salmonella is an important zoonotic pathogen and is a major cause of gastrointestinal diseases worldwide. The current serious problem of antibiotic abuse has prompted the search for new substitutes for antibiotics. JH-3 is a small antimicrobial peptide with broad-spectrum bactericidal activity. In this study, we showed that JH-3 has good bactericidal activity towards the clinical isolate Salmonella enterica serovar Typhimurium strain CVCC541. The minimum inhibitory concentration (MIC) of JH-3 against this bacterium was determined to be 100 μg/mL, which could decrease the number of CVCC541 cells by 1000-fold in vitro within 5 h. The transmission electron microscopy (TEM) results showed that JH-3 can damage the cell wall and membrane of CVCC541, leading to the leakage of cell contents and subsequent cell death. To measure the bactericidal activity of CVCC541-infected mice were treated intraperitoneally 40 or 10 mg/kg JH-3 at 2 h or 3 days postinfection. Our results showed that treatment with 40 mg/kg JH-3 at 2 h postinfection had the best therapeutic effect and could significantly protect mice from a lethal dose of CVCC541. Furthermore, the clinical symptoms, bacterial burden in blood and organs, and intestinal pathological changes were all decreased and were close to normal. This study examined the therapeutic effect of the antimicrobial peptide JH-3 against S. enterica CVCC541 infection for the first time and determined the therapeutic effect of different JH-3 doses and treatment times, laying the foundation for studies of new antimicrobial agents.

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http://link.springer.com/10.1007/s12602-019-09533-w
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http://dx.doi.org/10.1007/s12602-019-09533-wDOI Listing
April 2019
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