Genetic Diversity of the Pear Scab Fungus in Korea.

Authors:
Eu Ddeum Choi
Eu Ddeum Choi
Sunchon National University
Suncheon | South Korea
Gyoung Hee Kim
Gyoung Hee Kim
Sunchon National University
Suncheon | South Korea
Jang Hoon Song
Jang Hoon Song
National Institute of Horticultural and Herbal Science
Young Sun Lee
Young Sun Lee
Sunchon National University
Young Jin Koh
Young Jin Koh
Sunchon National University

Mycobiology 2019 Mar 1;47(1):76-86. Epub 2019 Mar 1.

Department of Plant Medicine, Sunchon National University, Suncheon, Korea.

Scab disease caused by is of agroeconomic importance in cultivation of Asian pear. However, little is known about the degree of genetic diversity in the populations of this pathogen. In this study, we collected 55 isolates from pear scab lesions in 13 major cultivation areas in Korea and examined the diversity using sequences of internal transcribed spacer (ITS) region, β-tubulin (TUB2), and translation elongation factor-1 (TEF-1) genes as molecular markers. Despite a low level of overall sequence variation, we found three distinctive subgroups from phylogenetic analysis of combined ITS, TUB2, and TEF-1 sequences. Among the three subgroups, subgroup 1 (60% of isolates collected) was predominant compared to subgroup 2 (23.6%) or subgroup 3 (16.4%) and was distributed throughout Korea. To understand the genetic diversity among the subgroups, RAPD analysis was performed. The isolates yielded highly diverse amplicon patterns and none of the defined subgroups within the dendrogram were supported by bootstrap values greater than 30%. Moreover, there is no significant correlation between the geographical distribution and the subgroups defined by molecular phylogeny. Our data suggest a low level of genetic diversification among the populations of in Korea.

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http://dx.doi.org/10.1080/12298093.2019.1572263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6452914PMC
March 2019
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