Multilocus Sequence Typing and Population Genetic Analysis of : Host Specificity and Its Impacts on Public Health.

Authors:
Wei Li
Wei Li
State Key Laboratory of Stem Cell and Reproductive Biology
China
Lihua Xiao
Lihua Xiao
National Center for Emerging and Zoonotic Infectious Diseases
China

Front Genet 2019 2;10:307. Epub 2019 Apr 2.

College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

Microsporidia comprise a large class of unicellular eukaryotic pathogens that are medically and agriculturally important, but poorly understood. There have been nearly 1,500 microsporidian species described thus far, which are variable in biology, genetics, genomics, and host specificity. Among those, is the well-known species responsible for the most recorded cases of human microsporidian affections. The pathogen can colonize a broad range of mammals and birds and most of the animals surveyed share some genotypes with humans, posing a threat to public health. Based on DNA sequence analysis of the ribosomal internal transcribed spacer (ITS) and phylogenetic analysis, several hundreds of genotypes have been defined and clustered into different genetic groups with varied levels of host specificity. However, single locus-based typing using ITS might have insufficient resolution to discriminate among isolates with complex genetic or hereditary characteristics and to assess the elusive reproduction or transmission modes of the organism, highlighting the need for exploration and application of multilocus sequence typing (MLST) and population genetic tools. The present review begins with a primer on microsporidia and major microsporidian species, briefly introduces the recent advances on ITS genotyping and phylogeny, summarizes recent MLST and population genetic data, analyzes the inter- and intragroup host specificity at the MLST level, and interprets the public health implications of host specificity in zoonotic or cross-species transmission of this ubiquitous fungus.

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2019.00307DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454070PMC
April 2019
2 Reads

Article Mentions


Provided by Crossref Event Data
twitter
Twitter:
April 6, 2019, 11:28 pm EST
twitter
Twitter:
April 6, 2019, 9:40 pm EST

Publication Analysis

Top Keywords

host specificity
20
public health
12
population genetic
12
multilocus sequence
8
microsporidian species
8
mlst population
8
sequence typing
8
host
5
genetic
5
specificity
5
locus-based typing
4
single locus-based
4
levels host
4
genetic groups
4
groups varied
4
varied levels
4
typing insufficient
4
specificity single
4
clustered genetic
4
genetic hereditary
4

Similar Publications