Isolation and characterisation of pVa-21, a giant bacteriophage with anti-biofilm potential against Vibrio alginolyticus.

Authors:
Sang Guen Kim
Sang Guen Kim
Laboratory of Aquatic Biomedicine
Jin Woo Jun
Jin Woo Jun
Seoul National University
Sib Sankar Giri
Sib Sankar Giri
Periyar Maniammai University
Thanjavur | India
Saekil Yun
Saekil Yun
Laboratory of Aquatic Biomedicine
Hyoun Joong Kim
Hyoun Joong Kim
Laboratory of Aquatic Biomedicine
Sang Wha Kim
Sang Wha Kim
College of Medicine
Jeong Woo Kang
Jeong Woo Kang
Konkuk University
Se Jin Han
Se Jin Han
Laboratory of Aquatic Biomedicine

Sci Rep 2019 Apr 18;9(1):6284. Epub 2019 Apr 18.

Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Republic of Korea.

There is an increasing emergence of antibiotic-resistant Vibrio alginolyticus, a zoonotic pathogen that causes mass mortality in aquatic animals and infects humans; therefore, there is a demand for alternatives to antibiotics for the treatment and prevention of infections caused by this pathogen. One possibility is through the exploitation of bacteriophages. In the present study, the novel bacteriophage pVa-21 was classified as Myoviridae and characterised as a candidate biocontrol agent against V. alginolyticus. Its morphology, host range and infectivity, growth characteristics, planktonic or biofilm lytic activity, stability under various conditions, and genome were investigated. Its latent period and burst size were estimated to be approximately 70 min and 58 plaque-forming units/cell, respectively. In addition, phage pVa-21 can inhibit bacterial growth in both the planktonic and biofilm states. Furthermore, phylogenetic and genome analysis revealed that the phage is closely related to the giant phiKZ-like phages and can be classified as a new member of the phiKZ-like bacteriophages that infect bacteria belonging to the family Vibrionaceae.

Download full-text PDF

Source
http://www.nature.com/articles/s41598-019-42681-1
Publisher Site
http://dx.doi.org/10.1038/s41598-019-42681-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472347PMC
April 2019
7 Reads

Publication Analysis

Top Keywords

vibrio alginolyticus
8
planktonic biofilm
8
activity stability
4
growth characteristics
4
lytic activity
4
biofilm lytic
4
stability conditions
4
characteristics planktonic
4
conditions genome
4
burst size
4
size estimated
4
period burst
4
latent period
4
investigated latent
4
genome investigated
4
infectivity growth
4
classified myoviridae
4
myoviridae characterised
4
pva-21 classified
4
bacteriophage pva-21
4

References

(Supplied by CrossRef)
Article in Aquac. Res.
K Sharma et al.
Aquac. Res. 2012
Article in Aquac. Res.
O Takaoka et al.
Aquac. Res. 2011
Article in Dis. Aquat. Organ.
C-H Liu et al.
Dis. Aquat. Organ. 2004
Article in Microbios.
PC Liu et al.
Microbios. 2001
Article in FAO Fisheries and aquaculture technical paper
K Cochrane et al.
FAO Fisheries and aquaculture technical paper 2009
Article in Nat. Clim. Change
C Baker-Austin et al.
Nat. Clim. Change 2013
Article in Atlantic. P. Natl. Acad. Sci. USA
L Vezzulli et al.
Atlantic. P. Natl. Acad. Sci. USA 2016
Article in ISME J.
NE Kimes et al.
ISME J. 2012
Article in Aquaculture
I Karunasagar et al.
Aquaculture 1996
Article in Appl. Environ. Microb.
L Coquet et al.
Appl. Environ. Microb. 2002
Article in Infect. Immun.
R Tamayo et al.
Infect. Immun. 2010

Similar Publications