Mouse ANKRD31 Regulates Spatiotemporal Patterning of Meiotic Recombination Initiation and Ensures Recombination between X and Y Sex Chromosomes.

Authors:
Frantzeskos Papanikos
Frantzeskos Papanikos
Laboratory of Analytical Chemistry
Erika Testa
Erika Testa
Institute Pasteur Cenci-Bolognetti
Ramya Ravindranathan
Ramya Ravindranathan
Indian Institute of Science Education and Research
Corinne Grey
Corinne Grey
Institut de Génétique Humaine
Montpellier | France
Ihsan Dereli
Ihsan Dereli
Institute of Physiological Chemistry

Mol Cell 2019 Jun 15;74(5):1069-1085.e11. Epub 2019 Apr 15.

Institute of Physiological Chemistry, Faculty of Medicine, Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany. Electronic address:

Orderly segregation of chromosomes during meiosis requires that crossovers form between homologous chromosomes by recombination. Programmed DNA double-strand breaks (DSBs) initiate meiotic recombination. We identify ANKRD31 as a key component of complexes of DSB-promoting proteins that assemble on meiotic chromosome axes. Genome-wide, ANKRD31 deficiency causes delayed recombination initiation. In addition, loss of ANKRD31 alters DSB distribution because of reduced selectivity for sites that normally attract DSBs. Strikingly, ANKRD31 deficiency also abolishes uniquely high rates of recombination that normally characterize pseudoautosomal regions (PARs) of X and Y chromosomes. Consequently, sex chromosomes do not form crossovers, leading to chromosome segregation failure in ANKRD31-deficient spermatocytes. These defects co-occur with a genome-wide delay in assembling DSB-promoting proteins on autosome axes and loss of a specialized PAR-axis domain that is highly enriched for DSB-promoting proteins in wild type. Thus, we propose a model for spatiotemporal patterning of recombination by ANKRD31-dependent control of axis-associated DSB-promoting proteins.

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Source
https://linkinghub.elsevier.com/retrieve/pii/S10972765193022
Publisher Site
http://dx.doi.org/10.1016/j.molcel.2019.03.022DOI Listing

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June 2019
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