Description of a Natural Infection with Decapod Iridescent Virus 1 in Farmed Giant Freshwater Prawn, .

Authors:
Liang Qiu
Liang Qiu
Jiangsu Provincial Geriatrics Hospital
China
Xing Chen
Xing Chen
Peking University
China
Chen Li
Chen Li
Catalytic Hydrogenation Research Center
China
Wen Gao
Wen Gao
Shanghai Pulmonary Hospital
China
Qing-Li Zhang
Qing-Li Zhang
Huazhong Agricultural University
China
Jie Huang
Jie Huang
St. Jude Children's Research Hospital
United States

Viruses 2019 Apr 17;11(4). Epub 2019 Apr 17.

Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences; Laboratory for Marine Fisheries Science and Food Production Processes, National Laboratory for Marine Science and Technology (Qingdao); Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs; Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Qingdao 266071, China.

is a valuable freshwater prawn in Asian aquaculture. In recent years, a new symptom that was generally called "white head" has caused high mortality in farms in China. Samples of , , , , , and Cladocera from a farm suffering from white head in Jiangsu Province were collected and analyzed in this study. Pathogen detection showed that all samples were positive for Decapod iridescent virus 1 (DIV1). Histopathological examination revealed dark eosinophilic inclusions and pyknosis in hematopoietic tissue, hepatopancreas, and gills of and . Blue signals of in situ digoxigenin-labeled loop-mediated isothermal amplification appeared in hematopoietic tissue, hemocytes, hepatopancreatic sinus, and antennal gland. Transmission electron microscopy of ultrathin sections showed a large number of DIV1 particles with a mean diameter about 157.9 nm. The virogenic stromata and budding virions were observed in hematopoietic cells. Quantitative detection with TaqMan probe based real-time PCR of different tissues in naturally infected showed that hematopoietic tissue contained the highest DIV1 load with a relative abundance of 25.4 ± 16.9%. Hepatopancreas and muscle contained the lowest DIV1 loads with relative abundances of 2.44 ± 1.24% and 2.44 ± 2.16%, respectively. The above results verified that DIV1 is the pathogen causing white head in . and are also species susceptible to DIV1.

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http://dx.doi.org/10.3390/v11040354DOI Listing
April 2019
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References

(Supplied by CrossRef)
Analysis of iridescent viral disease of shrimp (SHID) in 2017
Qiu et al.
2018
Viral infections of Macrobrachium spp.: Global status of outbreaks, diagnosis, surveillance, and research
Hameed et al.
Isr. J. Aquac. 2009

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