Molecular cloning and mRNA expression of IFIT5 in tissues of ducklings infected with virulent duck hepatitis A virus type 3.

Authors:
Xuelian Zhang
Xuelian Zhang
Fudan University
China
Wenjing Zhang
Wenjing Zhang
MOE Key Laboratory of Macromolecular Synthesis and Functionalization
China
Yue Liu
Yue Liu
Xiyuan Hospital
China
Haihui Qi
Haihui Qi
College of Veterinary Medicine
Chunxue Hao
Chunxue Hao
State Key Laboratory of Metastable Materials Science and Technology
Wenlong Zhang
Wenlong Zhang
College of Veterinary Medicine
China
Mingchun Gao
Mingchun Gao
College of Veterinary Medicine
China
Junwei Wang
Junwei Wang
College of Veterinary Medicine
China

Res Vet Sci 2019 Apr 8;124:256-262. Epub 2019 Apr 8.

Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, PR China; Northeastern Science Inspection Station, China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Northeast Agricultural University, Harbin 150030, PR China. Electronic address:

Interferon-induced proteins with tetratricopeptide repeats (IFITs) are a family of proteins strongly induced downstream of type I interferon signaling. The function of IFITs has been investigated extensively in mammals. IFIT5 is the sole protein in this family found in birds and little information is available about the function of avian IFIT5. In this study, duck IFIT5 was cloned from peripheral blood mononuclear cells. Multiple amino acid sequence alignment and phylogenetic analysis showed that duck IFIT5 is highly homologous to chicken IFIT5. Tissue specificity analysis demonstrated that duck IFIT5 was ubiquitously expressed in all examined tissues of five-day-old ducklings, with the highest expression levels in heart, followed by thymus, cerebrum, liver, and lung; kidney expressed the lowest. Quantitative real-time PCR (qRT-PCR) analysis revealed that duck IFIT5 expression rapidly increased both in vitro and in vivo after stimulation with polyinosinic:polycytidylic acid [poly (I:C)] and infection with virulent duck hepatitis A virus type 3 (DHAV-3), respectively. Altogether, these results indicate that the expression of duck IFIT5 is positively correlated with viral load and may play an important role in the immune response to DHAV-3 infection. This study lays a foundation for further research into the innate antiviral immune responses of ducklings.

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Source
https://linkinghub.elsevier.com/retrieve/pii/S00345288183132
Publisher Site
http://dx.doi.org/10.1016/j.rvsc.2019.04.003DOI Listing
April 2019
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