Dermatological Implications of Galectin-3 in Circulation: An Evaluation From the Perspective of Patients With Differential Manifestations of Post-Kala-Azar Dermal Leishmaniasis.

Authors:
Souvik Datta
Souvik Datta
Calcutta School of Tropical Medicine
Department of Laboratory Medicine
Research in glycoproteomics
Kolkata-700073, West Bengal | India
Manab Ghosh
Manab Ghosh
School of Tropical Medicine
Koushik Dewan
Koushik Dewan
Chittaranjan National Cancer Institute
Bibhuti Saha
Bibhuti Saha
Indian Institute of Chemical Biology
India
Sumi Mukhopadhyay
Sumi Mukhopadhyay
Indian Institute of Chemical Biology
India

Am J Dermatopathol 2019 Apr 10. Epub 2019 Apr 10.

Departments of Laboratory Medicine, and.

Galectin-3, a β-galactoside-binding lectin, has been implicated in vast repertoire of inflammatory and immunomodulatory processes including skin diseases. However, galectin-3 has not been comprehensively studied in infectious diseases. This study emphasizes on fascinating aspects of galectin-3 expression in dermal infection by studying post-kala-azar dermal leishmaniasis (PKDL), an intracellular infection caused by Leishmania donovani. Indian PKDL is a well-recognized parasitic dermatosis, with a high risk of anthroponotic transmission of L. donovani in causing leishmaniasis. This study aims to investigate the levels of galectin-3 and galectin-3-binding site expression in circulation of different forms of Indian patients with PKDL. Thirty-seven confirmed untreated PKDL patients, comprising 20 polymorphic and 17 macular PKDL manifestations, were evaluated for the levels of sera galectin-3 with respect to 28 age- and sex-matched healthy controls from endemic areas. Result shows a significant increment (P < 0.001) in circulatory galectin-3 levels in PKDL variants as compared to healthy controls. In addition, there were heightened levels of galectin-3 and galectin-3-binding sites on cellular infiltrates on lesional sites. Furthermore, there was a positive correlation between frequencies of mononuclear cells and galectin-3 during microcirculation in lesions. Data were well corroborated with positive correlation of IL-10 and IFN-γ with sera galectin-3 levels. Moreover, flow cytometry demonstrated the enhanced expression levels of the galectin-3-binding site in circulation in patients with PKDL as compared to healthy controls. Taken together, elevated levels of galectin-3 reflect its involvement in PKDL pathogenesis.

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http://dx.doi.org/10.1097/DAD.0000000000001412DOI Listing
April 2019
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