Detection of enzootic nasal tumor virus (ENTV) in a sheep flock in southern Brazil.

Trop Anim Health Prod 2019 Apr 17. Epub 2019 Apr 17.

Department of Veterinary Pathology, Veterinary School, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.

Enzootic nasal tumor (ENT) is a contagious neoplasm associated with enzootic nasal tumor virus (ENTV), which may induce disease in sheep (ENTV-1) and goats (ENTV-2). This study aimed to describe the occurrence of ENT in two Texel sheep (Ovis aries) from a 75-sheep flock, located in the city of Gravataí, southern Brazil. Animals used to be purchased from different origins, and no specific tests for disease monitoring or quarantine procedure were performed. Affected animals presented respiratory distress, anorexia with severe weight loss, and mucopurulent unilateral nasal discharge. Necropsy was performed in both animals and nasal cavity masses were observed. Histopathological analysis demonstrated an epithelial neoplasm compatible with nasal adenocarcinoma. PCR using a protocol that amplifies a 591 bp sequence of 5'LTR-gag region of ENTV1 was performed followed by DNA sequencing. Both samples were positive, and the sequences obtained presented highest identity (97%) with ENTV strain TN28 (GenBank accession number MH899613) detected in a Texel sheep from Scotland. This is the first report of ENTV-1 leading to enzootic nasal tumor in sheep in Latin America, which confirms the presence of the retrovirus in sheep flocks in the Brazilian territory.

Download full-text PDF

Source
http://link.springer.com/10.1007/s11250-019-01897-z
Publisher Site
http://dx.doi.org/10.1007/s11250-019-01897-zDOI Listing
April 2019
4 Reads

Publication Analysis

Top Keywords

enzootic nasal
16
nasal tumor
16
southern brazil
8
virus entv
8
texel sheep
8
performed animals
8
tumor virus
8
nasal
7
sheep
6
neoplasm compatible
4
epithelial neoplasm
4
masses observed
4
analysis demonstrated
4
histopathological analysis
4
observed histopathological
4
demonstrated epithelial
4
pcr protocol
4
amplifies 591 bp
4
591 bp sequence
4
sequence 5'ltr-gag
4

References

(Supplied by CrossRef)
Article in Veterinary Pathology.
M Las Heras De et al.
Veterinary Pathology. 1991
Article in Current Topics in Microbiology and Immunology
M las Heras De et al.
Current Topics in Microbiology and Immunology 2003
Article in Journal of Virology
M Kuprovic et al.
Journal of Virology 2018
Article in Small Ruminant Research
C Leroux et al.
Small Ruminant Research 2008

J Sambrook et al.
2001

JM Sharp et al.
2000
Article in Open Veterinary Journal
N Sid et al.
Open Veterinary Journal 2018
Article in Slovenian Veterinary Research
T Švara et al.
Slovenian Veterinary Research 2006
Article in Molecular Biology and Evolution
K Tamura et al.
Molecular Biology and Evolution 2013
Article in Virus Research
SR Walsh et al.
Virus Research 2010
Article in Journal of General Virology
SR Walsh et al.
Journal of General Virology 2014

Similar Publications