NDV-3A vaccination prevents C. albicans colonization of jugular vein catheters in mice.

Authors:
Shakti Singh
Shakti Singh
University of Alberta
Edmonton | Canada
John E Edwards
John E Edwards
David Geffen School of Medicine at UCLA
Ashraf S Ibrahim
Ashraf S Ibrahim
Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center
United States
Priya Uppuluri
Priya Uppuluri
The University of Texas at San Antonio
United States

Sci Rep 2019 Apr 17;9(1):6194. Epub 2019 Apr 17.

Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA, USA.

NDV-3A, a novel fungal vaccine undergoing clinical trials, contains a recombinant version of the Candida albicans rAls3 N-terminus protein (rAls3p-N) in aluminum hydroxide. In a Phase 1b/2a clinical trial, NDV-3A protected women from recurrent vulvovaginal candidiasis. Here, we reveal that active immunization in mice with NDV-3A induces high titers of anti-rAls3p-N antibodies that interfere with C. albicans ability to adhere to and invade endothelial cells, and form biofilm in vitro. Anti-rAls3p-N antibodies also significantly inhibit yeast dispersal from the hyphal layers of biofilms. Compared to placebo, NDV-3A vaccination inhibited C. albicans dissemination to kidneys and prevented colonization of central venous catheters in mice. Overall, these preclinical studies suggest that NDV-3A may serve as an immunotherapeutic strategy for prevention of infections on indwelling medical devices.

Download full-text PDF

Source
http://www.nature.com/articles/s41598-019-42517-y
Publisher Site
http://dx.doi.org/10.1038/s41598-019-42517-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6470131PMC

Still can't find the full text of the article?

We can help you send a request to the authors directly.
April 2019
9 Reads
5.078 Impact Factor

Publication Analysis

Top Keywords

mice ndv-3a
8
catheters mice
8
anti-rals3p-n antibodies
8
ndv-3a vaccination
8
ndv-3a
6
invade endothelial
4
adhere invade
4
ability adhere
4
albicans ability
4
endothelial cells
4
cells form
4
antibodies inhibit
4
inhibit yeast
4
vitro anti-rals3p-n
4
biofilm vitro
4
form biofilm
4
interfere albicans
4
women recurrent
4
active immunization
4
immunization mice
4

References

(Supplied by CrossRef)
Article in Crit Rev Microbiol
M Ilkit et al.
Crit Rev Microbiol 2011
Article in Journal of Clinical Microbiology
R. A. Hajjeh et al.
Journal of Clinical Microbiology 2004
Article in Clin Infect Dis
O Gudlaugsson et al.
Clin Infect Dis 2003
Article in Pathog Dis
C Tsui et al.
Pathog Dis 2016
Article in Journal of Oral Microbiology
David W. Williams et al.
Journal of Oral Microbiology 2013
Article in Annu Rev Microbiol
CJ Nobile et al.
Annu Rev Microbiol 2015
Article in PLoS Biol
QT Phan et al.
PLoS Biol 2007
Article in Microbiology
X Zhao et al.
Microbiology 2006
Article in PLoS Pathog
RS Almeida et al.
PLoS Pathog 2008
Article in Clin Infect Dis
JE Edwards Jr. et al.
Clin Infect Dis 2018

Similar Publications