Hospital-derived antibody profiles of malaria patients in Southwest India.

Authors:
Apoorva Venkatesh
Apoorva Venkatesh
Indian Institute of Technology Bombay
India
Aarti Jain
Aarti Jain
University of California
United States
Huw Davies
Huw Davies
Emory University
United States
Jennifer N Maki
Jennifer N Maki
University of Washington
Edwin Gomes
Edwin Gomes
Goa Medical College and Hospital
Philip L Felgner
Philip L Felgner
University of California
Sanjeeva Srivastava
Sanjeeva Srivastava
Indian Institute of Technology Bombay
India

Malar J 2019 Apr 17;18(1):138. Epub 2019 Apr 17.

Department of Chemistry and Department of Global Health, University of Washington, Seattle, WA, 98195, USA.

Background: Naturally acquired immunity to malaria across the globe varies in intensity and protective powers. Many of the studies on immunity are from hyperendemic regions of Africa. In Asia, particularly in India, there are unique opportunities for exploring and understanding malaria immunity relative to host age, co-occurrence of Plasmodium falciparum and Plasmodium vivax infections, varying travel history, and varying disease severity. Variation in immunity in hospital settings is particularly understudied.

Methods: A US NIH ICEMR (South Asia) team examined the level of immunity in an Indian malaria patient population visiting or admitted to Goa Medical College and Hospital in Goa, India. Sera from 200 patients of different ages, in different seasons, infected with P. falciparum or P. vivax or both species, and with different clinical severity were applied to an established protein array system with over 1000 P. falciparum and P. vivax antigens. Differential binding of patient IgG to different antigens was measured.

Results: Even though Goa itself has much more P. vivax than P. falciparum, IgG reactivity towards P. falciparum antigens was very strong and comparable to that seen in regions of the world with high P. falciparum endemicity. Of 248 seropositive P. falciparum antigens, the strongest were VAR, MSP10, HSP70, PTP5, AP2, AMA1, and SYN6. In P. vivax patients, ETRAMPs, MSPs, and ApiAP2, sexual stage antigen s16, RON3 were the strongest IgG binders. Both P. falciparum and P. vivax patients also revealed strong binding to new antigens with unknown functions. Seropositives showed antigens unique to the young (HSP40, ACS6, GCVH) or to non-severe malaria (MSP3.8 and PHIST).

Conclusion: Seroreactivity at a major hospital in Southwest India reveals antibody responses to P. falciparum and P. vivax in a low malaria transmission region with much migration. In addition to markers of transmission, the data points to specific leads for possible protective immunity against severe disease. Several, but not all, key antigens overlap with work from different settings around the globe and from other parts of India. Together, these studies confidently help define antigens with the greatest potential chance of universal application for surveillance and possibly for disease protection, in many different parts of India and the world.

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http://dx.doi.org/10.1186/s12936-019-2771-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472095PMC
April 2019
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References

(Supplied by CrossRef)
Article in Malar J.
MK Das et al.
Malar J. 2017
Article in J Vector Borne Dis.
BA Mannan et al.
J Vector Borne Dis. 2003
Article in Am J Trop Med Hyg.
SK Sharma et al.
Am J Trop Med Hyg. 2004
Article in Indonesian Bull Health Res.
JK Baird et al.
Indonesian Bull Health Res. 1991
Article in Nature
S Cohen et al.
Nature 1961
Article in J Exp Med
MF Muellenbeck et al.
J Exp Med 2013
Article in Am J Trop Med Hyg
A Sabchareon et al.
Am J Trop Med Hyg 1991
Article in PLoS ONE
GE Weiss et al.
PLoS ONE 2011
Article in Proc Natl Acad Sci USA
PD Crompton et al.
Proc Natl Acad Sci USA 2010
Article in Proteomics.
DL Doolan et al.
Proteomics. 2008
Article in PLoS Negl Trop Dis.
M Arévalo-Herrera et al.
PLoS Negl Trop Dis. 2016

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