Shear-induced alignment of collagen fibrils using 3D cell printing for corneal stroma tissue engineering.

Authors:
Hyeonji Kim
Hyeonji Kim
College of Applied Life Sciences
Jinah Jang
Jinah Jang
Sungkyunkwan University
South Korea
Kyoung-Pil Lee
Kyoung-Pil Lee
Pusan National University
Seunghun Lee
Seunghun Lee
Hanyang University Hospital
South Korea
Dong-Mok Lee
Dong-Mok Lee
Korea University
Ki Hean Kim
Ki Hean Kim
Massachusetts Institute of Technology
United States
Hong Kyun Kim
Hong Kyun Kim
Kyungpook National University School of Medicine
Winston-Salem | United States

Biofabrication 2019 May 7;11(3):035017. Epub 2019 May 7.

Department of Mechanical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Pohang, Gyeongbuk, 37673, Republic of Korea.

The microenvironments of tissues or organs are complex architectures comprised of structural proteins including collagen. Particularly, the cornea is organized in a lattice pattern of collagen fibrils which play a significant role in its transparency. This paper introduces a transparent bioengineered corneal structure for transplantation. The structure is fabricated by inducing shear stress to a corneal stroma-derived decellularized extracellular matrix bioink based on a 3D cell printing technique. The printed structure recapitulates the native macrostructure of the cornea with aligned collagen fibrils which results in the construction of a highly matured and transparent cornea stroma analog. The level of shear stress, controlled by the various size of the printing nozzle, manipulates the arrangement of the fibrillar structure. With proper parameter selection, the printed cornea exhibits high cellular alignment capability, indicating a tissue-specific structural organization of collagen fibrils. In addition, this structural regulation enhances critical cellular events in the assembly of collagen over time. Interestingly, the collagen fibrils that remodeled along with the printing path create a lattice pattern similar to the structure of native human cornea after 4 weeks in vivo. Taken together, these results establish the possibilities and versatility of fabricating aligned collagen fibrils; this represents significant advances in corneal tissue engineering.

Download full-text PDF

Source
http://iopscience.iop.org/article/10.1088/1758-5090/ab1a8b
Publisher Site
http://dx.doi.org/10.1088/1758-5090/ab1a8bDOI Listing
May 2019
4 Reads

Publication Analysis

Top Keywords

collagen fibrils
24
tissue engineering
8
cell printing
8
shear stress
8
lattice pattern
8
collagen
8
aligned collagen
8
fibrils
6
structure
5
cornea
5
nozzle manipulates
4
manipulates arrangement
4
arrangement fibrillar
4
printing nozzle
4
size printing
4
controlled size
4
high cellular
4
fibrillar structure
4
exhibits high
4
stress controlled
4

Similar Publications