Metabolic Fingerprinting of Chorionic Villous Samples in Normal Pregnancy and Chromosomal Disorders.

Authors:
Federica Murgia
Federica Murgia
University of Cagliari
Italy
Ambra Iuculano
Ambra Iuculano
Microcitemico Hospital
Italy
Cristina Peddes
Cristina Peddes
Department of Prenatal and Preimplantation Genetic Diagnosis and Fetal Therapy
Maria Laura Santoru
Maria Laura Santoru
University of Cagliari
Italy
Laura Tronci
Laura Tronci
University of Cagliari
Cagliari | Italy
Monica Deiana
Monica Deiana
Cittadella Universitaria SS 554
Italy
Luigi Atzori
Luigi Atzori
University of Cagliari
Italy
Giovanni Monni
Giovanni Monni
Università degli Studi di Cagliari
Italy

Prenat Diagn 2019 Apr 17. Epub 2019 Apr 17.

Department of Prenatal and Preimplantation Genetic Diagnosis and Fetal Therapy, Ospedale Pediatrico Microcitemico A.Cao, Cagliari, Italy.

Objective: Placenta-related biological samples are used in biomedical research to investigate placental development. Metabolomics represents a promising approach for studying placental metabolism in an effort to explain physiological and pathological mechanisms. The aim of this study was to investigate metabolic changes in chorionic villi during the first trimester of pregnancy in euploid and aneuploid cases.

Methods: Samples from 21 women (13 euploid, 8 aneuploid) were analyzed with H-Nuclear Magnetic Resonance (NMR), Gas Chromatography-Mass Spectrometry (GC-MS) and High-Performance Liquid chromatography (HPLC). Multivariate statistical analysis was performed and differences in metabolites were used to identify the altered metabolic pathways.

Results: A regression model to test the correlation between fetal crown-rump length (CRL) and metabolic profile of chorionic villous was performed in euploid pregnancies (R was 0.69 for the NMR analysis and 0.94 for the GC-MS analysis). Supervised Analysis was used to compare chorionic villi of euploid and aneuploid fetuses (NMR: R X=0.70, R Y=0.65, Q2=0.30 R X=0.62; GC-MS: R Y=0.704, Q =0.444). Polyol pathways, myo-inositol and oxidative stress seem to have a fundamental role in euploid and aneuploid pregnancies.

Conclusion: Polyol pathways may have a crucial role in energy production in early pregnancy. Excessive activation in aneuploid pregnancies may lead to increased oxidative stress. Metabolomics represents a promising approach to investigate placental metabolic changes.

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Source
http://dx.doi.org/10.1002/pd.5461DOI Listing
April 2019

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