Peptide-Directed DNA-Templated Protein Labelling for The Assembly of a Pseudo-IgM.

Authors:
Rasmus P Thomsen
Rasmus P Thomsen
Biomolecular Nanoscale Engineering Center and Interdisciplinary Nanoscience Center (iNANO)
Per F Nielsen
Per F Nielsen
United Arab Emirates University
United Arab Emirates
Thomas E Nielsen
Thomas E Nielsen
Carlsberg Laboratory
Denmark

Angew Chem Int Ed Engl 2019 Apr 17. Epub 2019 Apr 17.

Aarhus University, Centre for DNA Nanotechnology at the Dept. of Chemsitry and iNANO, Gustav Wieds Vej 14, 8000, Aarhus, DENMARK.

The development of methods for conjugation of DNA to proteins is of high relevance for integration of protein function and DNA structures. Here, we demonstrate that protein binding peptides can direct a DNA-templated reaction, furnishing DNA-protein conjugates selectively with one DNA label. Quantitative conversion of oligonucleotides is achieved at low stoichiometries and the reaction can be performed in complex biological matrixes such as cell lysates. Further, we have applied a star-like pentameric DNA nanostructure to assemble five DNA-Rituximab conjugates, made by our reported method, into a pseudo-IgM antibody structure that was subsequently characterized by negative stain transmission electron microscopy (nsTEM) analysis.

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http://dx.doi.org/10.1002/anie.201903134DOI Listing
April 2019
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