Production of l-Ribulose Using an Encapsulated l-Arabinose Isomerase in Yeast Spores.

Authors:
Xiaoxiao Liu
Xiaoxiao Liu
Cancer Center
Zijie Li
Zijie Li
and Institute of High Energy Physics
China
Zhou Chen
Zhou Chen
Peking University People's Hospital
China
Ning Wang
Ning Wang
School of Chinese Medicine
Denver | United States
Yahui Gao
Yahui Gao
Xiamen University
China
Hideki Nakanishi
Hideki Nakanishi
The University of Tokushima Graduate School
Xiao-Dong Gao
Xiao-Dong Gao
Hokkaido University
Japan

J Agric Food Chem 2019 May 16;67(17):4868-4875. Epub 2019 Apr 16.

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology , Jiangnan University , Wuxi , Jiangsu 214122 , People's Republic of China.

The rare sugar l-ribulose is produced from the abundant sugar l-arabinose by enzymatic conversion. An l-arabinose isomerase (AI) from Geobacillus thermodenitrificans was efficiently expressed and encapsulated in Saccharomyces cerevisiae spores. Deletion of the yeast OSW2 gene, which causes a mild defect in the integrity of the spore wall, substantially improved the activity of encapsulated AI, without damaging its superior enzymatic properties of thermostability, pH tolerance,and resistance toward SDS and proteinase treatments. In a 10 mL reaction, 100 mg of dry AI encapsulated in spores produced 250 mg of l-ribulose from 1 g of l-arabinose, indicating a 25% conversion rate. Notably, the product of l-ribulose was directly purified from the reaction solution with an approximately 91% recovery using a Ca ion exchange column. Our results describe not only a facile approach for the production of l-ribulose but also a useful strategy for the enzymatic conversion of rare sugars in "Izumoring".

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Source
http://pubs.acs.org/doi/10.1021/acs.jafc.9b00640
Publisher Site
http://dx.doi.org/10.1021/acs.jafc.9b00640DOI Listing
May 2019
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