Identification of Group 2 Innate Lymphoid Cells in Mouse Lung, Liver, Small Intestine, Bone Marrow, and Mediastinal and Mesenteric Lymph Nodes.

Authors:
Monica Romera-Hernandez
Monica Romera-Hernandez
Erasmus University Medical Center Rotterdam
Netherlands
Catherine A Steer
Catherine A Steer
University of British Columbia and Terry Fox Laboratory
Fumio Takei
Fumio Takei
Terry Fox Laboratory
Canada

Curr Protoc Immunol 2019 Apr 17:e73. Epub 2019 Apr 17.

Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.

Innate lymphoid cells (ILCs) are a heterogeneous family of lymphocytes that populate barrier and non-barrier tissues. ILCs regulate immune responses to pathogens and commensals but also sustain metabolic homeostasis, tissue remodeling after injury and establish dialogue with the nervous system. ILCs rapidly become activated in the absence of adaptive antigen receptors by responding to signaling molecules provided by hematopoietic or non-hematopoietic cells. Here we provide protocols designed for processing the lung, liver, small intestine, bone marrow, mediastinal and mesenteric lymph nodes in order to obtain a purified leukocyte fraction of cells, in which ILC2 enrichment is optimized. In addition, we describe in detail the methodologies used to activate ILC2s and the assays necessary for the detection of their effector cytokines. We highlight the differences in ILC2 characterization within distinct tissues that we have recently identified. © 2019 by John Wiley & Sons, Inc.

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Source
https://onlinelibrary.wiley.com/doi/abs/10.1002/cpim.73
Publisher Site
http://dx.doi.org/10.1002/cpim.73DOI Listing
April 2019
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