Angew Chem Int Ed Engl 2019 Apr 17. Epub 2019 Apr 17.
Conventional analytical methods based on single detection to determine one disease marker are usually not sufficient and accurate because the progression of disease generally involves multiple chemicals and biomolecules. The drive for simultaneous analysis of multiple targets, which plays a key role in both basic biomedical research and clinical applications, demands the development of multiplexed bioassays with high-throughput. In this minireview, we systematically summarize the recent progress of optical multiplexed analytical techniques to improve biomedical diagnostics, in which fluorescence and surface enhanced Raman scattering (SERS) with distinctive optical features are chosen as the main readout signals. Taking advantage of the multiplexed strategies in the biomedical field, a selection of recent contributions from biosensing of multiple chemicals and multicolor cellular tracking to in vivo multiplexed bioimaging are highlighted. Finally, we frame the future outlook of challenges and opportunities of multiplexed bioanalysis.