Increase in the diagnosis of mycobacteremia after the implementation of semi-automated blood culture at a Brazilian public health laboratory.

Rev Soc Bras Med Trop 2019 Apr 11;52:e20180267. Epub 2019 Apr 11.

Departamento de Biologia Médica, Seção de Micobactérias, Laboratório de Saúde Pública Dr. Giovanni Cysneiros, Goiânia, GO, Brasil.

Introduction: The prevalence of hematogenous dissemination of mycobacteria is high in immunosuppressed patients. The isolation of mycobacteria in culture remains the standard procedure.

Methods: This is a cross-sectional study based on the results of solid (Löwenstein-Jensen medium) and semi-automated liquid (BACTEC 9240) blood cultures, obtained from the Lacen-GO database.

Results: The implementation of a semi-automated procedure resulted in an increase of 61.5% and 350.0% in the positive results for Mycobacterium tuberculosis complex and nontuberculous mycobacteria, respectively. This technique also accelerated the detection of positive results.

Conclusions: Semi-automated liquid blood culture showed a better performance in the diagnosis of mycobacteremia.

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http://dx.doi.org/10.1590/0037-8682-0267-2018DOI Listing
April 2019
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References

(Supplied by CrossRef)

Grange JM et al.
2009

2015
Tuberculosis
Lawn SD et al.
Lancet 2011

Forbes BA et al.
2007
New and improved tuberculosis diagnostics: evidence, policy, practice, and impact
Pai M et al.
Curr Opin Pulm Med 2010
Liquid vs. solid culture for tuberculosis: performance and cost in a resource-constrained setting
Chihota VN et al.
Int J Tuberc Lung Dis 2010
Comparative evaluation of the BACTEC MGIT 960 system with solid medium for isolation of mycobacteria
Lee JJ et al.
Int J Tuberc Lung Dis 2003
Multicenter comparison of ESP Culture System II with BACTEC 460TB and with Löwenstein-Jensen medium for recovery of mycobacteria from different clinical specimens, including blood
Tortoli E et al.
J Clin Microbiol 1998

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