Overexpression of microRNA-190 inhibits migration, invasion, epithelial-mesenchymal transition, and angiogenesis through suppression of protein kinase B-extracellular signal-regulated kinase signaling pathway via binding to stanniocalicin 2 in breast cancer.

Authors:
Guiming Sun
Guiming Sun
Liaocheng People's Hospital
Meirong Liu
Meirong Liu
Beijing National Laboratory for Molecular Sciences
Beijing | China
Hui Han
Hui Han
Sun Yat-sen University Cancer Center
China

J Cell Physiol 2019 Apr 16. Epub 2019 Apr 16.

Department of Oncology, Liaocheng People's Hospital, Liaocheng, P.R. China.

Breast cancer (BC) is known as the most deadly cancer among females, worldwide. Despite the research advances in this regard, effective diagnosis and treatment still have a long way to go. In this study, our stance was to investigate the regulatory mechanism of miR-190 on epithelial-mesenchymal transition (EMT) and angiogenesis via mediation of protein kinase B (AKT)-extracellular signal-regulated kinase (ERK) signaling pathway by targeting stanniocalicin 2 (STC2) in BC. The BC gene chip was retrieved with differentially expressed genes (DEGs) obtained. MDA-MB-231 and T47D cell lines were selected and separately introduced with miR-190 inhibitors, activators, and small interfering RNAs with the intent of exploring the regulatory functions that miR-190 has shown while governing STC2 in BC. The regulatory effect of miR-190 on cell proliferation, migration, invasion, and angiogenesis was evaluated, followed by determination of AKT-ERK signaling pathway-related factors, EMT-related factors, and angiogenesis-related factors. The xenograft tumor of nude mice was also implemented for determining the change of tumor after transfection. The GSE26910 gene chip was obtained with STC2 being selected as the potential DEG. STC2 was the target gene of miR-190. The results showed that cells introduced with the miR-190 activators along with small interfering RNA-STC2 inhibited proliferation, invasion, migration, angiogenesis, as well as EMT. Moreover, the in vivo experiment also went on to confirm that the tumor volume had significantly increased in the nude mice along with an elevated expression of miR-190. Collectively, the findings suggested that overexpression of miR-190 inhibited EMT and angiogenesis by inactivating AKT-ERK signaling pathway via STC2 in BC.

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Source
http://dx.doi.org/10.1002/jcp.28409DOI Listing
April 2019

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STC2 promotes the epithelial‐mesenchymal transition of colorectal cancer cells through AKT‐ERK signaling pathways
Chen B. et al.
Oncotarget 2016

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