Treatment potential of pathogen-reactive antibodies sequentially purified from pooled human immunoglobulin.

Authors:
Mark Reglinski
Mark Reglinski
University of Edinburgh
United Kingdom
Shiranee Sriskandan
Shiranee Sriskandan
Imperial College London
United Kingdom

BMC Res Notes 2019 Apr 15;12(1):228. Epub 2019 Apr 15.

Faculty of Medicine, Imperial College London, Hammersmith Campus, Du Cane Road, London, W12 0NN, UK.

Objective: Intravenous immune globulin (IVIG), pooled from human blood, is a polyspecific antibody preparation that inhibits the super-antigenic proteins associated with streptococcal and staphylococcal toxic shock, and the Shiga toxin. In addition to this toxin-neutralising activity, IVIG contains other pathogen-reactive antibodies that may confer additional therapeutic benefits. We sought to determine if pathogen-reactive antibodies that promote opsonophagocytosis of different organisms can be sequentially affinity-purified from one IVIG preparation.

Results: Antibodies that recognise cell wall antigens of Streptococcus pyogenes, Staphylococcus aureus, and vancomycin-resistant enterococcus (VRE) were sequentially affinity-purified from a single preparation of commercial IVIG and opsonophagocytic activity was assessed using a flow cytometry assay of neutrophil uptake. Non-specific IgG-binding proteins were removed from the S. aureus preparations using an immobilised Fc fragment column, produced using IVIG cleaved with the Immunoglobulin G-degrading enzyme of S. pyogenes (IdeS). Affinity-purified anti-S. aureus and anti-VRE immunoglobulin promoted significantly higher levels of opsonophagocytic uptake by human neutrophils than IVIG when identical total antibody concentrations were compared, confirming activity previously shown for affinity-purified anti-S. pyogenes immunoglobulin. The opsonophagocytic activities of anti-S. pyogenes, anti-S. aureus, and anti-VRE antibodies that were sequentially purified from a single IVIG preparation were undiminished compared to antibodies purified from previously unused IVIG.

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http://dx.doi.org/10.1186/s13104-019-4262-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466806PMC
April 2019
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References

(Supplied by CrossRef)
Article in Autoimmun Rev
JF Seite et al.
Autoimmun Rev 2008
Article in Sci Rep
M Reglinski et al.
Sci Rep 2015
Article in Clin Diagn Lab Immunol
MG Mikolajczyk et al.
Clin Diagn Lab Immunol 2004
Article in Infection
FS Rossmann et al.
Infection 2015
Article in J Pediatr
S Ashkenazi et al.
J Pediatr 1988
Article in Sci Transl Med
BA Diep et al.
Sci Transl Med 2016
Article in Eur J Clin Microbiol Infect Dis
N Farag et al.
Eur J Clin Microbiol Infect Dis 2013
Article in Infect Drug Resist
MM Sallam et al.
Infect Drug Resist 2016
Article in Proc Natl Acad Sci U S A
MC Enright et al.
Proc Natl Acad Sci U S A 2002
Article in Microb Genom
DS Smith et al.
Microb Genom 2016
Article in J Microbiol Methods
M Reglinski et al.
J Microbiol Methods 2016

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