A new immunohistochemical method to evaluate the development of vestibular compensation after unilateral labyrinthectomy in rats.

Authors:
Kazunori Matsuda
Kazunori Matsuda
Yakult Central Institute for Microbiological Research
Tadashi Kitahara
Tadashi Kitahara
Osaka University
Japan
Taeko Ito
Taeko Ito
Niigata University
Japan
Munehisa Fukushima
Munehisa Fukushima
Kansai Rosai Hospital
Amagasaki | Japan
Junya Fukuda
Junya Fukuda
Gunma University Graduate School of Medicine
Go Sato
Go Sato
Nihon University Veterinary Research Center
Japan
Yoshiaki Kitamura
Yoshiaki Kitamura
Graduate School of Pharmaceutical Sciences
Koji Abe
Koji Abe
Graduate School of Medicine
Knoxville | United States

Acta Otolaryngol 2019 Jun 16;139(6):505-510. Epub 2019 Apr 16.

a Department of Otolaryngology , Institute of Biomedical Sciences, Tokushima University Graduate School , Tokushima , Japan.

Background: Unilateral labyrinthectomy (UL) causes the disappearance of ipsilateral medial vestibular nuclear (ipsi-MVe) activity and induces spontaneous nystagmus (SN), which disappears during the initial process of vestibular compensation (VC). Ipsi-MVe-activity restores in the late process of VC.

Objective: We evaluated the late process of VC after UL in rats and examined the effects of thioperamide (H3 antagonist) on VC.

Materials And Methods: MK801 (NMDA antagonist)-induced Fos-like immunoreactive (-LIR) neurons in contra-MVe, which had been suppressed by NMDA-mediated cerebellar inhibition in UL rats was used as an index.

Results: The number of MK801-induced Fos-LIR neurons in contra-MVe gradually decreased to the same level as that of sham-operated rats 14 days after UL. Thioperamide moved the disappearance of the MK801-induced Fos-LIR neurons 2 days earlier. The number of MK801-induced Fos-LIR neurons in thioperamide-treated rats was significantly decreased, compared with that of vehicle rats on days 7 and 12 after UL. But, thioperamide did not influence the decline of SN frequency in UL rats.

Conclusion: These findings suggested that the number of MK801-induced Fos-LIR neurons in contra-MVe was decreased in concordance with the restoration of ipsi-MVe-activity during the late process of VC after UL and that thioperamide accelerated the late, but not the initial process of VC.

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Source
http://dx.doi.org/10.1080/00016489.2019.1599140DOI Listing
June 2019

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