Fluorometric determination of HIV DNA using molybdenum disulfide nanosheets and exonuclease III-assisted amplification.

Authors:
Lele Wang
Lele Wang
Nankai University
China
Lianhua Dong
Lianhua Dong
China Agricultural University
China
Gang Liu
Gang Liu
Peking Union Medical College Hospital
China
Xizhong Shen
Xizhong Shen
Zhongshan Hospital
Jing Wang
Jing Wang
The University of Texas MD Anderson Cancer Center
Houston | United States
Changfeng Zhu
Changfeng Zhu
Shanghai Institute of Applied Physics
China
Min Ding
Min Ding
Chongqing Medical University
China
Yanli Wen
Yanli Wen
Shanghai Institute of Applied Physics
China

Mikrochim Acta 2019 Apr 15;186(5):286. Epub 2019 Apr 15.

Laboratory of Biometrology, Shanghai Institute of Measurement and Testing Technology, Shanghai, 201203, People's Republic of China.

A convenient and ultrasensitive fluorometric method is described for the determination of HIV DNA. It exploits the strong difference in the affinities of MoS nanosheets for long ssDNA versus short oligonucleotide fragments. In addition, efficient signal amplification is accomplished by exonuclease III-assisted target recycling. When absorbed on the MoS nanosheets, the fluorescence of the FAM-labeled ssDNA probe (FP) is quenched. However, in the presence of HIV DNA, the FP hybridizes with target to form a duplex. As a result, the FP in the duplex will be stepwise hydrolyzed into short fragments by Exo III, and the fluorescence signal thus is retained because short fragments have low affinity for the MoS nanosheets. By using the Exo III-assisted target recycling amplification, the detection sensitivity is strongly improved. The sensor can detect DNA in a concentration as low as 5.3 pM (at an S/N ratio of 3), and the analytical range extends from 0.01 nM to 10 nM. The assay is simple, sensitive and specific, and conceivably represents a valuable tool in clinical studies related to the HIV. Graphical abstract Schematic presentation of fluorometric determination of HIV DNA based on molybdenum disulfide nanosheets and Exo III. When the fluorescence-tagged ssDNA probe hybridized with target to form a duplex, the Exo III-assisted target recycling amplification is generated. The method can detect as low as 5.3 pM HIV DNA.

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http://dx.doi.org/10.1007/s00604-019-3368-yDOI Listing
April 2019
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References

(Supplied by CrossRef)
Article in Nat Genet
C Debouck et al.
Nat Genet 1999
Article in Biotechnol Adv
V Velusamy et al.
Biotechnol Adv 2010
Article in Biosens Bioelectron
BM Paddle et al.
Biosens Bioelectron 1996
Article in Anal Bioanal Chem
S Rodriguez-Mozaz et al.
Anal Bioanal Chem 2006
Article in Angew Chem, Int Ed Engl
KM Wang et al.
Angew Chem, Int Ed Engl 2009
Article in Science
YWC Cao et al.
Science 2002
Article in J Am Chem Soc
F Patolsky et al.
J Am Chem Soc 2000
Article in Anal Chem
C Larsson et al.
Anal Chem 2003
Article in Nat Biotechnol
F Patolsky et al.
Nat Biotechnol 2001
Article in Proc Natl Acad Sci U S A
CH Fan et al.
Proc Natl Acad Sci U S A 2003
Article in J Am Chem Soc
RH Yang et al.
J Am Chem Soc 2008

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