Fluorometric determination of cardiac myoglobin based on energy transfer from a pyrene-labeled aptamer to graphene oxide.

Authors:
Dongkui Liu
Dongkui Liu
Eshelman School of Pharmacy
Yanbo Zeng
Yanbo Zeng
East China Normal University
Guobao Zhou
Guobao Zhou
Shanghai Institute of Applied Physics
Xing Lu
Xing Lu
University of Tsukuba
Japan
Dongwei Miao
Dongwei Miao
College of Biological
Yiwen Yang
Yiwen Yang
Zhejiang University
China
Dr.radical Jian Zhang
Dr.radical Jian Zhang
Biomedical Polymers Laboratory
Shanghai Institute of Materia Medica
Shanghai, Shanghai | China

Mikrochim Acta 2019 Apr 15;186(5):287. Epub 2019 Apr 15.

College of Biological, Chemical Sciences and Engineering, Jiaxing University, 118 Jiahang Road, Jiaxing, 314001, Zhejiang, China.

The authors describe a fluorometric assay for cardiac myoglobin (Mb), a marker for myocardial infarction. An Mb-binding aptamer was labeled with pyrene and adsorbed on the surface of graphene oxide (GO) via noncovalent and reversible binding forces. This causes the fluorescence of pyrene (best measured at excitation/emission wavelengths of 275/376 nm) to be quenched. However, fluorescence is restored on addition of pyrene due to the strong affinity between Mb and aptamer which causes its separation from GO. Fluorescence increases linearly in the 5.6-450 pM Mb concentration range, and the lower detection limit is 3.9 pM (S/N = 3). The assay was applied to the determination of cardiac Mb in spiked serum, and satisfactory results were obtained. Graphical abstract Schematic presentation of the detection of Mb (cardiac myoglobin) by using a fluorometric method based on pyrene-modified anti-Mb aptamer and GO (graphene oxide) through fluorescence quenching and subsequent recovery.

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http://dx.doi.org/10.1007/s00604-019-3385-xDOI Listing
April 2019
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