Ratiometric fluorescent immunoassay for the cardiac troponin-I using carbon dots and palladium-iridium nanocubes with peroxidase-mimicking activity.

Authors:
Xiaofeng Tan
Xiaofeng Tan
Central South University of Forestry and Technology
China
Lianhua Zhang
Lianhua Zhang
Shanghai Jiaotong University
China
Gengxiu Zheng
Gengxiu Zheng
Shandong Provincial Key Laboratory of Fluorine Chemistry and Chemical Materials
He Li
He Li
Key Laboratory of Meat Processing and Quality Control
China

Mikrochim Acta 2019 Apr 13;186(5):280. Epub 2019 Apr 13.

School of Chemistry and Chemical Engineering, and Institute of Surface Analysis and Chemical Biology, University of Jinan, Jinan, 250022, China.

A nanozyme-linked immunosorbent assay is described for cardiac troponin I which is a biomarker for myocardial infarction. The method is based on the use of Pd-Ir nanocubes with excellent peroxidase-like activity. The nanocubes catalyze the oxidization of nonfluorescent o-phenylenediamine (OPD) by HO to form a yellow fluorescent product (oxOPD) with excitation/emission maxima at 400/570 nm. Carbon dots are added as a reference fluorophore. Under the same excitation wavelength, they display blue fluorescence (450 nm). The ELISA uses the Pd-Ir nanocubes as a label for the secondary antibody and OPD as substrate. The ratio of fluorescence intensities at 570 and 450 nm increases in the 1 pg·mL to 1 ng·mL cardiac troponin I concentration range, and the detection limit is 0.31 pg·mL. The method was applied to analyze spiked serum samples, and the results compared well with those obtained by a commercial chemiluminescence assay. Graphical abstract Schematic presentation of the ratiometric fluorescence immunoassay for cardiac troponin-I. Pd-Ir nanocubes were employed to fabricate nanozyme-based signal labels for its excellent peroxidase-mimicking activity.

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Source
http://dx.doi.org/10.1007/s00604-019-3375-zDOI Listing
April 2019
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