Stage-specific transcriptome of Bursaphelenchus xylophilus reveals temporal regulation of effector genes and roles of the dauer-like stages in the lifecycle.

Authors:
Suguru E Tanaka
Suguru E Tanaka
Laboratory of Forest Botany
Yasunobu Maeda
Yasunobu Maeda
Shizuoka University
Isheng J Tsai
Isheng J Tsai
Wellcome Trust Sanger Institute
United Kingdom
Ryusei Tanaka
Ryusei Tanaka
Saga University
Japan
Mark Bligh
Mark Bligh
School of Civil and Environmental Engineering
Kenji Fukuda
Kenji Fukuda
Obihiro University of Agriculture and Veterinary Medicine
Japan

Sci Rep 2019 Apr 15;9(1):6080. Epub 2019 Apr 15.

Division of Parasitology, Faculty of Medicine, University of Miyazaki, Miyazaki, 889-1692, Japan.

The pine wood nematode Bursaphelenchus xylophilus is the causal agent of pine wilt disease, one of the most devastating forest diseases in East Asian and West European countries. The lifecycle of B. xylophilus includes four propagative larval stages and gonochoristic adults which are involved in the pathogenicity, and two stages of dispersal larvae involved in the spread of the disease. To elucidate the ecological roles of each developmental stage in the pathogenic life cycle, we performed a comprehensive transcriptome analysis using RNA-seq generated from all developmental stages of B. xylophilus and compared transcriptomes between stages. We found more than 9000 genes are differentially expressed in at least one stage of the life cycle including genes involved in general nematode biology such as reproduction and moulting but also effector genes likely to be involved in parasitism. The dispersal-stage transcriptome revealed its analogy to C. elegans dauer and the distinct roles of the two larval stages from each other regarding survival and transmission. This study provides important insights and resources to understand B. xylophilus parasitic biology.

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Source
http://dx.doi.org/10.1038/s41598-019-42570-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6465311PMC
April 2019

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