J Dent 2019 Apr 13. Epub 2019 Apr 13.
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address:
Objectives: This study evaluated the staining potential of TiF varnish and TiF/NaF solution on both sound and previously eroded enamel in vitro.
Methods: One hundred and eighty bovine enamel samples were polished; half of them remained sound and the other half was eroded (4 × 90 s each, using a 0.1% citric acid). Thereafter, the baseline color reading was performed (T). The samples were then subdivided into the following treatments: TiF varnish (24,500 ppm F, 1 × 6 h), NaF varnish (24,500 ppm F, 1 × 6 h), placebo varnish (1 × 6 h), TiF/NaF solution (500 ppm F, 6 × 1 min), Erosion Protection-Elmex (500 ppm F, 6 × 1 min) and control (water, 6 × 1 min). Between the applications, the samples were exposed to artificial saliva for 30 min. The color changes were measured immediately after the applications (T-T) and after the exposure to artificial saliva (S-S) by using a spectrophotometer (Vita EasyShade). The ΔE, L*, a* and b* data were compared using 2-way ANOVA/Tukey (p < 0.05).
Results: TiF varnish induced significantly higher color change (ΔE) compared to NaF and placebo varnishes on sound enamel immediately after application, but not after saliva exposure. TiF/NaF solution induced lower ΔE values compared to control on sound enamel; however, no differences were found between TiF/NaF and Elmex. For eroded, no differences were seen between the tested varnishes and solutions. Both types of fluoride solutions increased the yellow appearance of enamel, while the varnishes did not.
Conclusions: The color changes induced by the experimental TiF products are similar to the commercial ones.
Clinical Significance: Both TiF varnish and TiF/NaF solution have shown staining potential similar to commercially available varnish (NaF) and solution (Elmex) on sound and eroded enamel. These findings support the conduction of clinical trials, which, in the future, may lead to the commercialization of these products.