Methyl ketone production by Pseudomonas putida is enhanced by plant-derived amino acids.

Authors:
Jie Dong
Jie Dong
Peking University First Hospital
China
Yan Chen
Yan Chen
Institute of Public Health
St. Louis | United States
Christopher J Petzold
Christopher J Petzold
Joint BioEnergy Institute
Emeryville | United States
Harry R Beller
Harry R Beller
Lawrence Livermore National Laboratory
Livermore | United States
Aymerick Eudes
Aymerick Eudes
Joint BioEnergy Institute
United States
Henrik V Scheller
Henrik V Scheller
University of Copenhagen
Denmark

Biotechnol Bioeng 2019 Apr 14. Epub 2019 Apr 14.

Joint BioEnergy Institute, Emeryville, California.

Plants are an attractive sourceof renewable carbon for conversion to biofuels and bio-based chemicals. Conversion strategies often use a fraction of the biomass, focusing on sugars from cellulose and hemicellulose. Strategies that use plant components, such as aromatics and amino acids, may improve the efficiency of biomass conversion. Pseudomonas putida is a promising host for its ability to metabolize a wide variety of organic compounds. P. putida was engineered to produce methyl ketones, which are promising diesel blendstocks and potential platform chemicals, from glucose and lignin-related aromatics. Unexpectedly, P. putida methyl ketone production using Arabidopsis thaliana hydrolysates was enhanced 2-5-fold compared with sugar controls derived from engineered plants that overproduce lignin-related aromatics. This enhancement was more pronounced (~seven-fold increase) with hydrolysates from nonengineered switchgrass. Proteomic analysis of the methyl ketone-producing P. putida suggested that plant-derived amino acids may be the source of this enhancement. Mass spectrometry-based measurements of plant-derived amino acids demonstrated a high correlation between methyl ketone production and amino acid concentration in plant hydrolysates. Amendment of glucose-containing minimal media with a defined mixture of amino acids similar to those found in the hydrolysates studied led to a nine-fold increase in methyl ketone titer (1.1 g/L).

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1002/bit.26995
Publisher Site
http://dx.doi.org/10.1002/bit.26995DOI Listing

Still can't find the full text of the article?

We can help you send a request to the authors directly.
April 2019
7 Reads

Publication Analysis

Top Keywords

amino acids
20
methyl ketone
16
ketone production
12
plant-derived amino
12
lignin-related aromatics
8
pseudomonas putida
8
amino
6
methyl
6
putida
5
acids
5
derived engineered
4
sugar controls
4
enhanced 2-5-fold
4
2-5-fold compared
4
compared sugar
4
engineered plants
4
controls derived
4
nonengineered switchgrass
4
pronounced ~seven-fold
4
~seven-fold increase
4

References

(Supplied by CrossRef)
Opportunities and challenges in biological lignin valorization
Beckham G. T. et al.
Current Opinion in Biotechnology 2016
Synthesis of three advanced biofuels from ionic liquid‐pretreated switchgrass using engineered Escherichia coli
Bokinsky G. et al.
Proceedings of the National Academy of Sciences of the United States of America 2011
The emerging role for bacteria in lignin degradation and bio‐product formation
Bugg T. D. H. et al.
Current Opinion in Biotechnology 2011
Chemicals from biomass: Technologicalversus environmental feasibility. A review
Fiorentino G. et al.
Biofuels, Bioproducts and Biorefining 2017

Similar Publications