A sensitive scoring system for the longitudinal clinical evaluation and prediction of lethal disease outcomes in newborn mice.

Authors:
Beate Fehlhaber
Beate Fehlhaber
Hannover Medical School
Germany
Anna S Heinemann
Anna S Heinemann
Hannover Medical School
Maike Willers
Maike Willers
Universitätsklinikum Erlangen and Friedrich-Alexander Universität (FAU) Erlangen-Nürnberg
Nuremberg | Germany
Sandra Pfeifer
Sandra Pfeifer
University of Veterinary Medicine Hannover
Dorothee Viemann
Dorothee Viemann
Hannover Medical School
Hannover | Germany

Sci Rep 2019 Apr 11;9(1):5919. Epub 2019 Apr 11.

Department of Paediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625, Hannover, Germany.

Neonatal animal models are increasingly employed in order to unravel age-specific disease mechanisms. Appropriate tools objectifying the clinical condition of murine neonates are lacking. In this study, we tested a scoring system specifically designed for newborn mice that relies on clinical observation and examination. Both, in a neonatal sepsis model and an endotoxic shock model, the scoring results strongly correlated with disease-induced death rates. Full as well as observation-restricted scoring, reliably predicted fatality and the remaining time until death. Clinical scores even proved as more sensitive biomarker than 6 traditionally used plasma cytokine levels in detecting sepsis at an early disease stage. In conclusion, we propose a simple scoring system that detects health impairments of newborn mice in a non-invasive longitudinal and highly sensitive manner. Its usage will help to meet animal welfare requirements and might improve the understanding of neonatal disease mechanisms.

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Source
http://www.nature.com/articles/s41598-019-42414-4
Publisher Site
http://dx.doi.org/10.1038/s41598-019-42414-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459866PMC
April 2019
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