Effects of vitamin D3 and its chemical analogs on the growth of Hodgkin's lymphoma, in vitro.

Authors:
Rajendra Gharbaran
Rajendra Gharbaran
Hackensack University Medical Center
United States
Bo Zhang
Bo Zhang
The Second Affiliated Hospital of Dalian Medical University
Dalian Shi | China
Luis Valerio
Luis Valerio
College Park
United States
Madeline Wong
Madeline Wong
National Cancer Institute
United States
Stephen Redenti
Stephen Redenti
Harvard Medical School
United States

BMC Res Notes 2019 Apr 8;12(1):216. Epub 2019 Apr 8.

Department of Biological Sciences, Lehman College, City University of New York, Bronx, NY, 10468, USA.

Objective: Vitamin D receptor (VDR) activities have been noted for a number of B cell malignancies which showed varying sensitivities to vitamin D3 (1,25-dihydroxyvitamin D3, VD3, calcitriol) and its synthetic analogs. The objective of this study was to address the potential effects of VD3 and vitamin D3 analogs (VDAs) on the growth of Hodgkin's lymphoma (HL), a malignant pathology of B cell origin, in vitro.

Results: Immunofluorescence staining showed the expression of VDR by primary Hodgkin's (H) and Reed-Sternberg (RS)-HRS-tumor cells in HL histological sections. Western blot analyses revealed expression of VDR in the HL cell lines Hs445, HDLM2, KMH2, and L428. One-way analysis of variance (ANOVA) on data obtained from water-soluble tetrazolium 1 (WST-1) cell proliferation assay showed decreased cell growth in HDLM2 and L428, 72 h after treatment with 10 µM of either VD3 of VDAs. Western blot analyses showed that treatment of L428 cells with the VDAs (calcipotriol and EB1089) resulted in modest increases in nuclear accumulation of VDR (nuVDR) compared to either dimethyl sulfoxide (DMSO) or VD3 treatments. nuVDR for DMSO control and VD3 was comparable. These results suggest that VD3 or VDAs may affect growth of HL.

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http://dx.doi.org/10.1186/s13104-019-4241-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454773PMC
April 2019
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References

(Supplied by CrossRef)
Article in Eur J Haematol
PM Kulling et al.
Eur J Haematol 2017
Article in J Clin Oncol
MT Drake et al.
J Clin Oncol 2010
Article in Cancer
IM Shui et al.
Cancer 2015
Article in Br J Cancer
Y Kim et al.
Br J Cancer 2014
Article in Breast Cancer
M Atoum et al.
Breast Cancer 2017
Article in Nutr Rev
S Samuel et al.
Nutr Rev 2008
Article in BMC Cancer
C Renne et al.
BMC Cancer 2012
Article in J Hematol Oncol
R Gharbaran et al.
J Hematol Oncol 2013
Article in Clin Exp Immunol
R Consolini et al.
Clin Exp Immunol 2001
Article in Br J Cancer
T Hickish et al.
Br J Cancer 1993
Article in J Steroid Biochem Mol Biol
P Kozielewicz et al.
J Steroid Biochem Mol Biol 2016

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