Mutation of oppF gene in the Mycoplasma synoviae MS-H vaccine strain and its implication for differential serological responses to vaccination versus field challenge.

Authors:
Somayeh Kordafshari
Somayeh Kordafshari
University of Tehran
Iran
Marc S Marenda
Marc S Marenda
The University of Melbourne
Australia
Pollob Shil
Pollob Shil
The University of Melbourne
Australia
Amir H Noormohammadi
Amir H Noormohammadi
The University of Melbourne
Australia

Vet Microbiol 2019 Apr 22;231:48-55. Epub 2019 Feb 22.

Asia Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Veterinary & Agricultural Sciences, The University of Melbourne, Werribee, Victoria, 3030, Australia.

Mycoplasma synoviae (MS) is a major pathogen of poultry globally, causing chronic respiratory disease and arthritis. Vaccination is an effective means for the control of the disease. The MS-H vaccine is an attenuated strain developed through chemical mutagenesis of an Australian field strain, 86079/7NS. Analysis of whole genome of MS-H and its comparison with that of 86079/7NS has revealed a frameshift mutation early in a gene (oppF) that codes for an oligopeptide transporter permease, OppF. Monospecific antibodies raised against peptides upstream and downstream of the mutation in OppF revealed that only N-terminus of the OppF was expressed in MS-H while the full version was expressed in 86079/7NS. Also, examination of the recombinant N- (OppF-N) and C termini (OppF-C) of OppF, upstream and downstream of the mutation site respectively, as well as the full length OppF in Western immunoblotting experiments showed that serum from MS-H vaccinated chicken strongly bound OppF-N while serum from 86079/7NS challenged chicken detected OppF, OppF-N and OppF-C. The potential of the recombinant OppF, OppF-N and OppF-C to discriminate antibody responses to MS-H reisolates with wild or vaccine type OppF was assessed against 88 chicken sera in indirect ELISA and ratios were calculated between optical densities (OD) over those obtained in MS major membrane protein MSPB ELISA. Comparison of the OD ratios revealed that the MSPB/OppF and MSPB/OppF-C OD ratios of the sera against isolates with vaccine type OppF were significantly higher than those against isolates with wild type OppF. These results are in accordance with oppF gene mutation in MS-H and confirms that MS-H does not express OppF beyond the frame shift mutation found in its oppF gene. Also, the indirect ELISA based on OppF-C in combination with the MSPB has the potential to differentiate between MS-H and field strain antibody responses.

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Source
https://linkinghub.elsevier.com/retrieve/pii/S03781135183108
Publisher Site
http://dx.doi.org/10.1016/j.vetmic.2019.02.029DOI Listing
April 2019
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