Genotyping Mycoplasma gallisepticum by multilocus sequence typing.

Authors:
Zsuzsa Kreizinger
Zsuzsa Kreizinger
Institute for Veterinary Medical Research
Hungary
Kinga M Sulyok
Kinga M Sulyok
Institute for Veterinary Medical Research
Hungary
Salvatore Catania
Salvatore Catania
viale dell'Università
Italy
Janet Bradbury
Janet Bradbury
University of Liverpool
United Kingdom
Inna Lysnyansky
Inna Lysnyansky
Kimron Veterinary Institute
Israel

Vet Microbiol 2019 Apr 15;231:191-196. Epub 2019 Mar 15.

Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária körút 21, Budapest 1143, Hungary; Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Hungária körút 23-25, Budapest 1143, Hungary. Electronic address:

Mycoplasma gallisepticum causes chronic respiratory disease and reproductive disorders in many bird species, resulting in considerable economic losses to the poultry industry. Maintenance of M. gallisepticum-free flocks is the most adequate method to control infection. To this end, monitoring systems and vaccination programs with live vaccine strains are applied worldwide. There is strong demand for efficient epidemiological investigation tools to distinguish M. gallisepticum strains in order to control disease. Up to now, multilocus sequence typing (MLST) has been regarded as gold standard for genotyping bacteria due to its good reproducibility and high discriminatory power. The aim of this study was to develop an MLST assay which can determine phylogenetic distances between M. gallisepticum strains. After analysing more than 30 housekeeping genes, six loci (atpG, dnaA, fusA, rpoB, ruvB, uvrA) were selected for the MLST assay due to their genomic location and high diversity. Examination of 130 M. gallisepticum strains with this MLST method yielded 57 unique sequence types (STs) with a 0.96 Simpson's index of diversity. Considering the large number of STs and high diversity index, this MLST method was found to be appropriate to discriminate M. gallisepticum strains. In addition, the developed method was shown to be suitable for epidemiological investigations, as it confirmed linkage between related strains from outbreaks in different farms. Besides, MLST also suggested high impact of extensive international trade on the spread of different M. gallisepticum strains. Furthermore this method can be used for differentiation among vaccine and field strains.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.vetmic.2019.03.016DOI Listing
April 2019
3 Reads

Publication Analysis

Top Keywords

gallisepticum strains
20
multilocus sequence
8
high diversity
8
sequence typing
8
strains
8
mlst assay
8
mycoplasma gallisepticum
8
mlst method
8
gallisepticum
7
mlst
6
method
5
atpg dnaa
4
dnaa fusa
4
method differentiation
4
loci atpg
4
genes loci
4
strains analysing
4
analysing housekeeping
4
housekeeping genes
4
fusa rpob
4

Similar Publications