Identification and characterization of Paracoccidioides lutzii proteins interacting with macrophages.

Authors:
Mariana Vieira Tomazett
Mariana Vieira Tomazett
Laboratório de Biologia Molecular
Lilian Cristiane Baeza
Lilian Cristiane Baeza
State University of Maringá
Brazil
Juliano Domiraci Paccez
Juliano Domiraci Paccez
Federal University of Rio Grande do Sul
Brazil
Fatima Ribeiro-Dias
Fatima Ribeiro-Dias
Radboud University Nijmegen Medical Centre
Netherlands

Microbes Infect 2019 Apr 2. Epub 2019 Apr 2.

Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001-970, Goiânia, Goiás, Brazil. Electronic address:

Paracoccidioidomycosis (PCM), caused by thermodimorphic fungi of the Paracoccidioides genus, is a systemic disorder that involves the lungs and other organs. The adherence of pathogenic microorganisms to host tissues is an essential event in the onset of colonization and spread. The host-pathogen interaction is a complex interplay between the defense mechanisms of the host and the efforts of pathogenic microorganisms to colonize it. Therefore, the identification of fungi proteins interacting with host proteins is an important step understanding the survival strategies of the fungus within the host. In this paper, we used affinity chromatography based on surface proteomics (ACSP) to investigate the interactions of pathogen proteins with host surface molecules. Paracoccidioides lutzii extracts enriched of surface proteins were captured by chromatographic resin, which was immobilized with macrophage cell surface proteins, and identified by mass spectrometry. A total of 215 proteins of P. lutzii were identified interacting with macrophage proteins. In silico analysis classified those proteins according to the presence of sites for N- and O-glycosylation and secretion by classical and non-classical pathways. Serine proteinase (SP) and fructose-1,6-bisphosphate aldolase (FBA) were identified in our proteomics analysis. Immunolocalization assay and flow cytometry both showed an increase in the expression of these two proteins during host-pathogen interaction.

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http://dx.doi.org/10.1016/j.micinf.2019.03.002DOI Listing
April 2019
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