Afferent neuropeptide Y projections to the ventral tegmental area in normal-weight male Wistar rats.

J Comp Neurol 2019 Apr 5. Epub 2019 Apr 5.

Department of Endocrinology and Metabolism & Laboratory of Endocrinology, Department of Clinical Chemistry, Amsterdam Neuroscience, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.

The hypothalamic neuropeptide Y (NPY) circuitry is a key regulator of feeding behavior. NPY also acts in the mesolimbic dopaminergic circuitry, where it can increase motivational aspects of feeding behavior through effects on dopamine output in the nucleus accumbens (NAc) and on neurotransmission in the ventral tegmental area (VTA). Endogenous NPY in the NAc originates from local interneurons and afferent projections from the hypothalamic arcuate nucleus (Arc). However, the origin of endogenous NPY in the VTA is unknown. We determined, in normal-weight male Wistar rats, if the source of VTA NPY is local, and/or whether it is derived from VTA-projecting neurons. Immunocytochemistry, in situ hybridization and RT-qPCR were utilized, when appropriate in combination with colchicine treatment or 24 hr fasting, to assess NPY/Npy expression locally in the VTA. Retrograde tracing using cholera toxin beta (CTB) in the VTA, fluorescent immunocytochemistry and confocal microscopy were used to determine NPY-immunoreactive afferents to the VTA. NPY in the VTA was observed in fibers, but not following colchicine pretreatment. No NPY- or Npy-expressing cell bodies were observed in the VTA. Fasting for 24 hr, which increased Npy expression in the Arc, failed to induce Npy expression in the VTA. Double-labeling with CTB and NPY was observed in the Arc and in the ventrolateral medulla. Thus, VTA NPY originates from the hypothalamic Arc and the ventrolateral medulla of the brainstem in normal-weight male Wistar rats. These afferent connections link hypothalamic and brainstem processing of physiologic state to VTA-driven motivational behavior.

Download full-text PDF

Source
http://dx.doi.org/10.1002/cne.24698DOI Listing

Still can't find the full text of the article?

We can help you send a request to the authors directly.
April 2019
3 Reads

Article Mentions


Provided by Crossref Event Data
twitter
Twitter:
April 20, 2019, 6:30 am EST
twitter
Twitter:
April 19, 2019, 8:18 am EST
twitter
Twitter:
April 19, 2019, 8:08 am EST

Publication Analysis

Top Keywords

normal-weight male
12
wistar rats
12
male wistar
12
vta npy
12
vta
10
npy
10
feeding behavior
8
npy expression
8
endogenous npy
8
ventrolateral medulla
8
arc ventrolateral
8
tegmental area
8
npy vta
8
ventral tegmental
8
cholera toxin
4
toxin beta
4
source vta
4
tracing cholera
4
local and/or
4
vta retrograde
4

References

(Supplied by CrossRef)
The neuropeptide Y/agouti gene‐related protein (AGRP) brain circuitry in normal, anorectic, and monosodium glutamate‐treated mice
Broberger C. et al.
Proceedings of the National Academy of Sciences of the United States of America 1998
Antibodies to small transmitter molecules and peptides: Production and application of antibodies to dopamine, serotonine, GABA, vasopressin, vasoactive intestinal peptide, neuropeptide Y, somsatostatin and substance P
Buijs R. M. et al.
Biomedical Research 1989
Anatomy and regulation of the central melanocortin system
Cone R. D. et al.
Nature Neuroscience 2005

Similar Publications