Blood cell parameters for screening and diagnosis of hereditary spherocytosis.

Authors:
Lin Liao
Lin Liao
Shandong University
China
Yuchan Xu
Yuchan Xu
The First Affiliated Hospital of Guangxi Medical University
Nanning | China
Hongying Wei
Hongying Wei
Peking University School of Public Health
China
Yuling Qiu
Yuling Qiu
Dong-A University
Wenqiang Chen
Wenqiang Chen
Xuanwu Hospital
China
Jian Huang
Jian Huang
Affiliated Hospital of Guizhou Medical University
Guiyang Shi | China
Yifeng Tao
Yifeng Tao
National Energy R&D Center for Biorefinery
Xuelian Deng
Xuelian Deng
The First Affiliated Hospital of Guangxi Medical University
Nanning Shi | China

J Clin Lab Anal 2019 May 3;33(4):e22844. Epub 2019 Apr 3.

Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Background: There is currently no single index for the diagnostic screening of hereditary spherocytosis (HS). However, hematology analyzers are widely used in hospital laboratories because of their highly automated performance and quality control procedure, and detection of some blood cell parameters may be useful for the early screening of HS.

Methods: We investigated the values of blood cell parameters for the screening and differential diagnosis of HS. We performed a descriptive study of 482 samples (67 cases of HS, 59 cases of G6PD deficiency, 57 cases of AIHA, 199 cases of thalassemia, and 100 cases of healthy controls) that were run on Beckman Coulter LH780 Hematology Analyzer.

Results: HS was characterized by increased MCHC, decreased MRV, MSCV-MCV < 0, and increased Ret with no concomitant increase in IRF. The areas under the ROC curves were MSCV-MCV (0.97; 95% CI 0.95-1.0) > MRV (0.94; 95% CI 0.91-0.97) > MCHC (0.92; 95% CI 0.88-0.97) > Ret/IRF (0.77; 95% CI 0.7-0.84). MSCV-MCV ≤ 0.6 fl was valuable parameter for the diagnostic screening of HS, with a sensitivity of 95.5% and specificity of 94.9%.

Conclusion: These indices have high reference values for differentiating HS from thalassemia, AIHA, and G6PD deficiency.

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http://dx.doi.org/10.1002/jcla.22844DOI Listing

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May 2019
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