Simultaneous detection of Dengue virus, Chikungunya virus, Zika virus, Yellow fever virus and West Nile virus.

Authors:
Jose A Boga
Jose A Boga
Hospital Universitario Central de Asturias
Mercedes Rodriguez
Mercedes Rodriguez
Marie Curie Research Institute
United Kingdom
Santiago Melon
Santiago Melon
Laboratorio de Virología

J Virol Methods 2019 Jun 28;268:53-55. Epub 2019 Mar 28.

Servicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.

Although certain mosquito-borne virus, such as Dengue virus (DENV), Chikungunya virus (CHIKV), Zika virus (ZIKV), Yellow fever virus (YFV) and West Nile virus (WNV), are an important public health concern in those countries where transmitter mosquitoes are endemic, several cases of travelers from those endemic countries have been recently reported in Europe. Thus, early diagnosis of these viruses is essential for patient management and adoption of preventive measures. An assay for the simultaneous detection of DENV, CHIKV, ZIKV, YFV and WNV based on a multiplex real-time (RT)-PCR and its usefulness for diagnosis in infection screenings and surveillance of arbovirus in non-endemic countries are described.

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http://dx.doi.org/10.1016/j.jviromet.2019.03.014DOI Listing
June 2019
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