Genetic and chemical approaches for surface charge engineering of enzymes and their applicability in biocatalysis: A review.

Authors:
Ye Zhou
Ye Zhou
Fudan University
China
Zheng Guo
Zheng Guo
University of Electronic Science and Technology of China
China
Bianca Perez
Bianca Perez
University of Central Florida
United States

Biotechnol Bioeng 2019 Jul 24;116(7):1795-1812. Epub 2019 Apr 24.

Agrotech, Danish Technological Institute, Aarhus, Denmark.

Surface charge engineering has received considerable interest from the scientific and industrial community in the last few decades. Although it was previously hypothesized that the surface charge-charge interactions were not a fundamental force to determine protein folding and stability, many studies today show that surface charge plays a key role determining protein structure and activity. This review aims to (a) highlight the value of surface charged engineering of proteins to improve enzyme stability and activity in aqueous media and in the presence of ionic liquids (ILs) and organic solvents, (b) describe the existing approaches (genetic engineering or chemical modifications) for surface charged engineering, and (c) demonstrate the applicability of these surface charged enzymes in biocatalysis. The review provides a new foundation for the scientific and research community to exploit the surface engineering of protein concept for the development of new enzymes that are more active and stable in the presence of ILs and organic solvents, thereby offering new opportunities for industrial biocatalysis. Furthermore, this review is a useful tool for researchers to decide the best available technology to improve their enzyme system/process.

Download full-text PDF

Source
http://dx.doi.org/10.1002/bit.26979DOI Listing
July 2019

Publication Analysis

Top Keywords

surface charge
12
surface charged
12
biocatalysis review
12
charged engineering
8
ils organic
8
surface
8
improve enzyme
8
organic solvents
8
charge engineering
8
engineering
6
proteins improve
4
review aims
4
enzyme stability
4
engineering proteins
4
presence ils
4
highlight surface
4
aims highlight
4
activity review
4
protein structure
4
plays key
4

References

(Supplied by CrossRef)

Similar Publications