in four captive kangaroos ( spp.) in China: Isolation of a strain of a new genotype from an eastern grey kangaroo ().

Authors:
Ruijing Su
Ruijing Su
Donghua University
China
Hui Dong
Hui Dong
Second Military Medical University
China
Tongyi Li
Tongyi Li
Zhengzhou Zoo
Zhengzhou Shi | China
Ziguo Yuan
Ziguo Yuan
Veterinary Institute
China
Chunlei Su
Chunlei Su
Washington University School of Medicine
United States
Dr. Longxian Zhang, PhD
Dr. Longxian Zhang, PhD
Henan Agricultural University
Distinguished professor
Veterinary parasitology
Zhengzhou, Henan | China
Yurong Yang
Yurong Yang
University of Arkansas
Fayetteville | United States

Int J Parasitol Parasites Wildl 2019 Apr 12;8:234-239. Epub 2019 Mar 12.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR China.

Marsupials are highly susceptible to infection. Here, we report infection in four kangaroos from a zoo in China. Kangaroos were imported into China in 2000 and were since bred in zoo. In 2017-2018, four kangaroos died due to respiratory system disease or injury. The bodies were submitted to the laboratory to test for infection. Antibodies to were found in 75% (3/4) of the kangaroos via the modified agglutination test with the cut-off 1:25. Cysts were observed in the histopathological sections of tongue and diaphragm or squashes of fresh myocardium in two kangaroos. These cysts were confirmed as by immunohistochemical staining and molecular biological analysis. One viable strain was isolated from one kangaroo and designated as TgRooCHn1. DNA from tachyzoites obtained from cell culture was characterized by 10 PCR-RFLP markers and the virulence genes ROP5 and ROP18. The genotype of this isolate did not match with any known genotypes; it was designated as ToxoDB#292. The virulence of TgRooCHn1 (10 tachyzoites) was non-lethal to mice, and it formed tissue cysts. To our knowledge, the present study is the first isolation of ToxoDB#292 strain from kangaroo. Improvemets for captive settings were initiated, including greater attention being paied to birds and stray cats, fed frozen meat for carnivores.

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Source
https://linkinghub.elsevier.com/retrieve/pii/S22132244193001
Publisher Site
http://dx.doi.org/10.1016/j.ijppaw.2019.03.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423483PMC

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April 2019
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