AA16, a new lytic polysaccharide monooxygenase family identified in fungal secretomes.

Authors:
David Navarro
David Navarro
Hospital Clínico Universitario
Spain
Mireille Haon
Mireille Haon
Universités de Provence et de la Méditerranée
France
Sacha Grisel
Sacha Grisel
Centre National de la Recherche Scientifique and Universités d'Aix-Marseille I et II
France
Didier Chevret
Didier Chevret
INRA
France
Mathieu Fanuel
Mathieu Fanuel
INRA
France
Bernard Henrissat
Bernard Henrissat
Aix-Marseille Université
France

Biotechnol Biofuels 2019 16;12:55. Epub 2019 Mar 16.

1Biodiversité et Biotechnologie Fongiques, UMR1163, INRA, Aix Marseille Université, Marseille, France.

Background: Lignocellulosic biomass is considered as a promising alternative to fossil resources for the production of fuels, materials and chemicals. Efficient enzymatic systems are needed to degrade the plant cell wall and overcome its recalcitrance. A widely used producer of cellulolytic cocktails is the ascomycete , but this organism secretes a limited set of enzymes. To improve the saccharification yields, one strategy is to upgrade the enzyme cocktail with enzymes produced by other biomass-degrading filamentous fungi isolated from biodiversity.

Results: In this study, the enzymatic cocktails secreted by five strains from the genus ( strains BRFM 405, 1487, 1489, 1490 and strain BRFM 430) were tested for their ability to boost a reference cocktail for the saccharification of pretreated biomass. Proteomic analysis of fungal secretomes that significantly improved biomass degradation showed that the presence of proteins belonging to a putative LPMO family previously identified by genome analysis and awaiting experimental demonstration of activity. Members of this novel LPMO family, named AA16, are encountered in fungi and oomycetes with life styles oriented toward interactions with plant biomass. One AA16 protein from (AaAA16) was produced to high level in LPMO-type enzyme activity was demonstrated on cellulose with oxidative cleavage at the C1 position of the glucose unit. AaAA16 LPMO was found to significantly improve the activity of CBHI on cellulosic substrates.

Conclusions: Although spp. has been investigated for decades for their CAZymes diversity, we identified members of a new fungal LPMO family using secretomics and functional assays. Properties of the founding member of the AA16 family characterized herein could be of interest for use in biorefineries.

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http://dx.doi.org/10.1186/s13068-019-1394-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420742PMC
March 2019
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