Subspecies Typing of Based on Ribosomal Subunit Protein Mass Variation by MALDI-TOF MS.

Authors:
Julian Rothen
Julian Rothen
Ifakara Health Institute
Tanzania
Frederic Foucault
Frederic Foucault
Institute for Experimental Oncology
Germany
Jochen Blom
Jochen Blom
Justus-Liebig-University Giessen
Gießen | Germany
Dulmini Nanayakkara
Dulmini Nanayakkara
Postgraduate Institute of Science
Carmen Li
Carmen Li
University of Alberta Edmonton
Canada
Margaret Ip
Margaret Ip
The Chinese University of Hong Kong
Hong Kong
Marcel Tanner
Marcel Tanner
Swiss Tropical and Public Health Institute
Switzerland

Front Microbiol 2019 11;10:471. Epub 2019 Mar 11.

Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute (Swiss TPH) Basel, Basel, Switzerland.

A ribosomal subunit protein (rsp)-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method was developed for fast subspecies-level typing of (Group B , GBS), a major cause of neonatal sepsis and meningitis. A total of 796 GBS whole genome sequences, covering the genetic diversity of the global GBS population, were used to predict molecular mass variability of 28 rsp and to identify unique rsp mass combinations, termed "rsp-profiles". The established GBS typing scheme was validated by MALDI-TOF MS analysis of GBS isolates at two independent research sites in Europe and South East Asia. We identified 62 rsp-profiles, with the majority (>80%) of the 796 GBS isolates displaying one of the six rsp-profiles 1-6. These dominant rsp-profiles classify GBS strains in high concordance with the core-genome based phylogenetic clustering. Validation of our approach by in-house MALDI-TOF MS analysis of 248 GBS isolates and external analysis of 8 GBS isolates showed that across different laboratories and MALDI-TOF MS platforms, the 28 rsp were detected reliably in the mass spectra, allowing assignment of clinical isolates to rsp-profiles at high sensitivity (99%) and specificity (97%). Our approach distinguishes the major phylogenetic GBS genotypes, identifies hyper-virulent strains, predicts the probable capsular serotype and surface protein variants and distinguishes between GBS genotypes of human and animal origin. We combine the information depth of whole genome sequences with the highly cost efficient, rapid and robust MALDI-TOF MS approach facilitating high-throughput, inter-laboratory, large-scale GBS epidemiological and clinical studies based on pre-defined rsp-profiles.

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2019.00471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421976PMC
March 2019
2 Reads

Publication Analysis

Top Keywords

gbs isolates
16
gbs
12
ribosomal subunit
8
796 gbs
8
gbs genotypes
8
maldi-tof analysis
8
genome sequences
8
analysis gbs
8
subunit protein
8
maldi-tof
6
isolates
5
mass
5
rsp-profiles
5
isolates displaying
4
displaying rsp-profiles
4
>80% 796
4
rsp-profiles majority
4
majority >80%
4
strains high
4
distinguishes gbs
4

Similar Publications