Profiling of the Causative Bacteria in Infected Lymphocysts after Lymphadenectomy for Gynecologic Cancer by Pyrosequencing the 16S Ribosomal RNA Gene Using Next-Generation Sequencing Technology.

Authors:
Yuya Nogami
Yuya Nogami
School of Medicine
Japan
Kouji Banno
Kouji Banno
School of Medicine
Japan
Masataka Adachi
Masataka Adachi
Kumamoto University Graduate School of Medical Sciences
Japan
Yusuke Kobayashi
Yusuke Kobayashi
School of Medicine
Japan
Eiichiro Tominaga
Eiichiro Tominaga
Keio University
Japan
Daisuke Aoki
Daisuke Aoki
School of Medicine
Japan

Infect Dis Obstet Gynecol 2019 21;2019:9326285. Epub 2019 Feb 21.

Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo 160-8582, Japan.

Background: Surgery for gynecologic cancer with lymphadenectomy and pelvic radiotherapy can produce lymphoceles that sometimes complicate with infection, resulting in abscesses. The true pathogenic bacteria of abscesses are not always found because of false-negative results due to administered antibiotics and difficulty with detection, including for anaerobic bacteria. Analyzing bacteria flora by next-generation sequencing (NGS) using 16S ribosomal DNA may reveal the true pathogenic bacteria in abscesses. This is the first report on causative pathogens for infectious lymphocele using this technology.

Methods: The subjects were patients who developed infectious lymphocele after surgery for gynecologic cancer at our hospital from July 2015 to September 2016. NGS analyses of bacterial flora were performed using specimens preserved at -80°C. Two steps of PCR were performed for purified DNA samples to obtain sequence libraries. Processing of sequence data, including operational taxonomic unit (OTU) definition, taxonomy assignment, and an OTU BLAST search were performed. All patients gave written informed consent and the study was approved by the institutional research ethics committee.

Results: Six patients underwent puncture and drainage. The result in most cases indicated a single causative pathogen, including and . NGS revealed that the causative bacteria in lymphocele infection are normally a single strain, such as a surface Gram-positive coccus or enteric bacteria. Antibiotics should be chosen as appropriate for elimination of these respective bacteria.

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Source
http://dx.doi.org/10.1155/2019/9326285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409011PMC
February 2019
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References

(Supplied by CrossRef)
Article in National Medical Journal of China
National Medical Journal of China 2017

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