Characterization of lymphocyte subsets in peripheral blood cells of children with EV71 infection.

Authors:
Ming-Qi Zhao
Ming-Qi Zhao
Guangzhou women and Children's Medical Center
Li-Hua Wang
Li-Hua Wang
Nankai University
China
Ying-Hua Li
Ying-Hua Li
Chungbuk National University
South Korea
Min Guo
Min Guo
The Scripps Research Institute
United States
Yi Chen
Yi Chen
West China Hospital
China
Xiao-Min Liu
Xiao-Min Liu
The First Clinical Hospital of Harbin Medical University
China

J Microbiol Immunol Infect 2019 Mar 14. Epub 2019 Mar 14.

Virus Laboratory, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 318 Renminzhong Road Yuexiu District, Guangzhou, 510120, China. Electronic address:

Background: Enterovirus 71 (EV71) is one of the major causative pathogens of hand, foot, and mouth disease (HFMD). Immune cells play a critical role in determining the outcomes of virus infection. We aimed to characterize the lymphocyte subsets and transcriptional levels of T lymphocytes-associated transcription factors in peripheral blood cells of children with EV71 infection.

Methods: Peripheral blood samples from 32 children with EV71 infection and 32 control subjects were included in this study. The frequencies of T-, B-lymphocytes, and their subsets were determined by flow cytometry. The expression of transcription factors, including T-bet, Gata3, ROR γ t, Foxp3, TCF-1, and BCL-6 in the whole blood cells were evaluated by real-time reverse-transcription quantitative polymerase chain reaction (RT-qPCR).

Results: The frequencies of T cells, helper T cells (Th), cytotoxic T cells (Tc), IFN-γ Th1, IFN-γ Tc1, and regulatory T (Treg) cells were significantly decreased (P < 0.01) in children with EV71 infection. As for IL-4 Th2, IL-4 Tc2, IL-17 Th17, IL-17 Tc17, follicular helper T cells (Tfh), CD3CD8IL-21 T cells, CD19 B cells, and CD19IL-10 B10 cells, their frequencies were significantly increased in the EV71 group (P < 0.01). The EV71 group had lower mRNA expressions of T-bet, Gata3, and Foxp3 than the control group (P < 0.05), whereas the expressions of ROR γ t, TCF-1, and BCL-6 showed no significant difference between two groups.

Conclusions: EV71 infection in children caused a decreased frequency of total Th, Tc and Treg cells, and increased percentages of B cell, Th2 and Th17 cells in blood.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jmii.2019.03.001DOI Listing
March 2019
3 Reads

Publication Analysis

Top Keywords

blood cells
12
peripheral blood
12
children ev71
12
cells children
8
lymphocyte subsets
8
transcription factors
8
cells
8
ev71 infection
8
levels lymphocytes-associated
4
frequencies cells
4
transcriptional levels
4
cytometry expression
4
factors including
4
lymphocytes-associated transcription
4
factors peripheral
4
virus infection
4
flow cytometry
4
subsets transcriptional
4
cells helper
4
infection aimed
4

Similar Publications